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Catalog Number Size Price
LS-C287203-10 10 µl $295 
LS-C287203-100 100 µl (0.2 mg/ml) $514 
HIF1A / HIF1 Alpha Antibody - Detection of Human HIF1 alpha by Immunocytochemistry. Sample: Formaldehyde-fixed asynchronous HeLa cells treated with cobalt chloride-200 mcM (left) or mock treatment (right). Antibody: Affinity purified rabbit anti-HIF1 alpha used at a dilution of 1:100 (2 ug/ml). Detection: Red-fluorescent Goat anti-Rabbit IgG-heavy and light chain cross-adsorbed Antibody DyLight 594 Conjugated.

Polyclonal Rabbit anti‑Human HIF1A / HIF1 Alpha Antibody (aa775‑C‑term, IHC, IF, WB) LS‑C287203

Polyclonal Rabbit anti‑Human HIF1A / HIF1 Alpha Antibody (aa775‑C‑term, IHC, IF, WB) LS‑C287203

Antibody:
HIF1A / HIF1 Alpha Rabbit anti-Human Polyclonal (aa775-C-term) Antibody
Application:
IHC, ICC, IF, WB, IP
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$295
LS-C287203-10
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
HIF1A / HIF1 Alpha Rabbit anti-Human Polyclonal (aa775-C-term) Antibody
Application:
IHC, ICC, IF, WB, IP
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
HIF1 Alpha antibody LS-C287203 is an unconjugated rabbit polyclonal antibody to HIF1 Alpha (HIF1A) (aa775-C-term) from human. It is reactive with human and mouse. Validated for ICC, IF, IHC, IP and WB. Cited in 1 publication.
Target
Human HIF1A / HIF1 Alpha
Synonyms
HIF1A | ARNT-interacting protein | ARNT interacting protein | BHLHe78 | HIF1 Alpha | Hypoxia-inducible factor1alpha | HIF-1A | HIF1 | MOP1 | Member of PAS superfamily 1 | PASD8 | HIF-1-alpha | HIF-1alpha | HIF1-ALPHA | Member of PAS protein 1
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Predicted
Rat, Bovine, Chicken (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
A portion of GeneID: 3091 corresponding to amino acids between 775 and the C-Terminus of UniprotID: Q16665
Epitope
aa775-C-term
Specificity
Region between residues 775 and the C-terminus (residue 826) of human hypoxia-inducible factor 1, alpha subunit using the numbering given in Swiss-Prot entry Q16665 (GeneID 3091).
Applications
  • IHC (1:100 - 1:500)
  • ICC (1:50 - 1:200)
  • Immunofluorescence
  • Western blot (1:2000 - 1:10000)
  • Immunoprecipitation (2 - 10 µg/mg lysate)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
IHC: Epitope retrieval with citrate buffer pH6.0 for 20 minutes using a pressure cooker is recommended for FFPE tissue sections. IHC-IF: 1:100-1:500. Epitope retrieval with citrate buffer pH 6.0 for 20 minutes using a pressure cooker is recommended for FFPE tissue sections.
Manufacturer
Bethyl Laboratories, Inc.
Presentation
TBS, 0.09% Sodium Azide, 0.1% BSA
Storage
Store at 2-8°C for up to one year.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About HIF1A / HIF1 Alpha
Q16665 NM_001530 NP_001521.1

LSBio Ratings

HIF1A / HIF1 Alpha Antibody (aa775-C-term) for IHC, ICC, IF/Immunofluorescence, WB/Western, IP LS-C287203 has an LSBio Rating of
Publications (4)

Publications (1)

Quantum Blue Reduces the Severity of Woody Breast Myopathy via Modulation of Oxygen Homeostasis-Related Genes in Broiler Chickens. Greene E, Flees J, Dadgar S, Mallmann B, Orlowski S, Dhamad A, Rochell S, Kidd M, Laurendon C, Whitfield H, Brearley C, Rajaram N, Walk C, Dridi S. Frontiers in physiology. 2019 October;10:

Customer Reviews (0)


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Requested From: United States
Date Requested: 4/27/2024