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Catalog Number Size Price
LS-C290487-10 10 µl (1000 µg/ml) $295 
LS-C290487-100 100 µl (1000 µg/ml) $514 
H2AFX / H2AX Antibody - Detection of mouse H2AX by immunohistochemistry. Sample: FFPE section of mouse CT26 colon carcinoma. Antibody: Affinity purified goat anti- H2AX used at a dilution of 1:1,000 (1µg/ml). Detection: DAB
H2AFX / H2AX Antibody - Detection of human H2AX by immunohistochemistry. Sample: FFPE section of human ovarian cancer. Antibody: Affinity purified goat anti- H2AX used at a dilution of 1:5,000 (0.2µg/ml). Detection: DAB
H2AFX / H2AX Antibody - Detection of Human H2AX by Immunohistochemistry. Sample: FFPE section of human lung cancer. Antibody: Affinity purified rabbit anti-H2AX used at a dilution of 1:5000 (0.2 ug/ml). Detection: DAB.
H2AFX / H2AX Antibody - Detection of Mouse H2AX by Immunohistochemistry. Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-H2AX used at a dilution of 1:1000 (1 ug/ml). Detection: DAB.
H2AFX / H2AX Antibody - Detection of human H2AX by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, and K-562cells prepared using RIPA lysis buffer. Antibody: Affinity purified rabbit anti-H2AX antibody used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
H2AFX / H2AX Antibody - Detection of Human H2AX by Western Blot. Samples: Whole cell lysate from 293T (15 and 50 ug) cells. Antibodies: Affinity purified goat anti-H2AX antibody used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
H2AFX / H2AX Antibody - Detection of mouse H2AX by immunohistochemistry. Sample: FFPE section of mouse CT26 colon carcinoma. Antibody: Affinity purified goat anti- H2AX used at a dilution of 1:1,000 (1µg/ml). Detection: DAB
H2AFX / H2AX Antibody - Detection of human H2AX by immunohistochemistry. Sample: FFPE section of human ovarian cancer. Antibody: Affinity purified goat anti- H2AX used at a dilution of 1:5,000 (0.2µg/ml). Detection: DAB
H2AFX / H2AX Antibody - Detection of Human H2AX by Immunohistochemistry. Sample: FFPE section of human lung cancer. Antibody: Affinity purified rabbit anti-H2AX used at a dilution of 1:5000 (0.2 ug/ml). Detection: DAB.
H2AFX / H2AX Antibody - Detection of Mouse H2AX by Immunohistochemistry. Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-H2AX used at a dilution of 1:1000 (1 ug/ml). Detection: DAB.
H2AFX / H2AX Antibody - Detection of human H2AX by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, and K-562cells prepared using RIPA lysis buffer. Antibody: Affinity purified rabbit anti-H2AX antibody used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
H2AFX / H2AX Antibody - Detection of Human H2AX by Western Blot. Samples: Whole cell lysate from 293T (15 and 50 ug) cells. Antibodies: Affinity purified goat anti-H2AX antibody used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
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Polyclonal Goat anti‑Human H2AFX / H2AX Antibody (C‑Terminus, IHC, WB) LS‑C290487

Polyclonal Goat anti‑Human H2AFX / H2AX Antibody (C‑Terminus, IHC, WB) LS‑C290487

Antibody:
H2AFX / H2AX Goat anti-Human Polyclonal (C-Terminus) Antibody
Application:
IHC, WB
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$295
LS-C290487-10
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
H2AFX / H2AX Goat anti-Human Polyclonal (C-Terminus) Antibody
Application:
IHC, WB
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
H2AX antibody LS-C290487 is an unconjugated goat polyclonal antibody to H2AX (H2AFX) (C-Terminus) from human. It is reactive with human and mouse. Validated for IHC and WB.
Target
Human H2AFX / H2AX
Synonyms
H2AFX | H2AX | Histone H2A.x | H2A.X | H2A histone family, member X | H2AX histone
Host
Goat
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
A portion of GeneID: 3014 corresponding to amino acids near the C-Terminus of UniprotID: P16104
Epitope
C-Terminus
Specificity
The epitope recognized by A303-837A maps to the C-terminus of human Histone H2AX using the numbering given in entry P16104 (GeneID 3014).
Applications
  • IHC (1:1000 - 1:5000)
  • Western blot (1:2000 - 1:10000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Failed Applications
  • Immunoprecipitation
Usage
IHC: Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Manufacturer
Bethyl Laboratories, Inc.
Presentation
Tris-citrate/phosphate buffer, pH 7-8, 0.09% sodium azide.
Storage
Store at 2-8°C for up to one year.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About H2AFX / H2AX
P16104 NM_002105 NP_002096.1

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Requested From: United States
Date Requested: 4/29/2024