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Antibodies > GPR49 / LGR5 > LS-C797459
 
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Catalog Number Size Price
LS-C797459-100 100 µg $511 
GPR49 / LGR5 Antibody - Figure from citation: Positive correlation between LSD1, c-Myc, ß-catenin and LGR5 expression in human colorectal tumor tissues. Expression levels of LSD1, LGR5, ß-catenin and c-Myc in consecutive sections from normal colon, adenoma and CRC tumor tissues. Dilution: 1:100
GPR49 / LGR5 Antibody - Figure from citation: Positive correlation between LSD1, c-Myc, ß-catenin and LGR5 expression in human colorectal tumor tissues. Expression levels of LSD1, LGR5, ß-catenin and c-Myc in consecutive sections from normal colon, adenoma and CRC tumor tissues. Dilution: 1:100
GPR49 / LGR5 Antibody - Figure from citation: Immunohistochemical staining of Lgr5 in normal gastric tissues. The normal gastric tissue, 40×magnified. (A) and 400× magnified. (B). Yellowspots represent the Lgr5 positive cells that are localized only at base of the glands in normal gastric mucosa. The arrows in the pictures show the immunoreactive cells stained in yellow color.
GPR49 / LGR5 Antibody - Figure from citation: Immunohistochemical staining of Lgr5 in normal gastric tissues. The normal gastric tissue, 40×magnified. (A) and 400× magnified. (B). Yellowspots represent the Lgr5 positive cells that are localized only at base of the glands in normal gastric mucosa. The arrows in the pictures show the immunoreactive cells stained in yellow color.
GPR49 / LGR5 Antibody - Figure from citation: Immunohistochemical analysis of Lgr5 expression in human normal colon biopsies; Lgr5+ cells are localized at the crypt base, where bona fide stem-like cells home; magnification is indicated in the boxes. Dilution: 1:200
GPR49 / LGR5 Antibody - Figure from citation: Immunohistochemical analysis of Lgr5 expression in human normal colon biopsies; Lgr5+ cells are localized at the crypt base, where bona fide stem-like cells home; magnification is indicated in the boxes. Dilution: 1:200
GPR49 / LGR5 Antibody - Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-LGR5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min,
GPR49 / LGR5 Antibody - Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-LGR5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min,
GPR49 / LGR5 Antibody - Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-LGR5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min,
GPR49 / LGR5 Antibody - Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-LGR5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min,
GPR49 / LGR5 Antibody - Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-LGR5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min,
GPR49 / LGR5 Antibody - Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-LGR5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min,
GPR49 / LGR5 Antibody - Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-LGR5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min,
GPR49 / LGR5 Antibody - Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-LGR5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min,
GPR49 / LGR5 Antibody - Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-LGR5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min,
GPR49 / LGR5 Antibody - Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-LGR5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min,
GPR49 / LGR5 Antibody - Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-LGR5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min,
GPR49 / LGR5 Antibody - Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-LGR5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min,
GPR49 / LGR5 Antibody - Figure from citation: [Nature] Cells expressing the stem cell marker Lgr5 was detected by immunostaining of hCECs in 2D culture. <br />Pubmed id: 27398792<br />Memo: Human; IF
GPR49 / LGR5 Antibody - Figure from citation: Immunocytochemistry of epithelium stem cell phenotype. Isolated dental epithelium following 5-day culture, overwhelmingly expressed Lgr5. Dilution: 1:100
GPR49 / LGR5 Antibody - Figure from citation: Immunocytochemistry of epithelium stem cell phenotype. Isolated dental epithelium following 5-day culture, overwhelmingly expressed Lgr5. Dilution: 1:100
GPR49 / LGR5 Antibody - Figure from citation: [Nature] Cells expressing the stem cell marker Lgr5 was detected by immunostaining of hCECs in 2D culture. <br />Pubmed id: 27398792<br />Memo: Human; IF
GPR49 / LGR5 Antibody - Anti-LGR5 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY LGR5.
GPR49 / LGR5 Antibody - Anti-LGR5 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY LGR5.
GPR49 / LGR5 Antibody - Immunofluorescent staining of HT29 cells using anti-LGR5 mouse monoclonal antibody green). Actin filaments were labeled with TRITC-phalloidin. (red), and nuclear with DAPI. (blue).
GPR49 / LGR5 Antibody - Immunofluorescent staining of HT29 cells using anti-LGR5 mouse monoclonal antibody green). Actin filaments were labeled with TRITC-phalloidin. (red), and nuclear with DAPI. (blue).
GPR49 / LGR5 Antibody - HEK293T cells were transfected with the pCMV6-ENTRY control. (Left lane) or pCMV6-ENTRY LGR5. (Right lane) cDNA for 48 hrs and lysed
GPR49 / LGR5 Antibody - HEK293T cells were transfected with the pCMV6-ENTRY control. (Left lane) or pCMV6-ENTRY LGR5. (Right lane) cDNA for 48 hrs and lysed
GPR49 / LGR5 Antibody - Immunoprecipitation(IP) of LGR5 by using TrueMab monoclonal anti-LGR5 antibodies. (Negative control: IP without adding anti-LGR5 antibody.). For each experiment, 500ul of DDK tagged LGR5 overexpression lysates. (at 1:5 dilution with HEK293T lysate), 2ug of anti-LGR5 antibody and 20ul. (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
GPR49 / LGR5 Antibody - Immunoprecipitation(IP) of LGR5 by using TrueMab monoclonal anti-LGR5 antibodies. (Negative control: IP without adding anti-LGR5 antibody.). For each experiment, 500ul of DDK tagged LGR5 overexpression lysates. (at 1:5 dilution with HEK293T lysate), 2ug of anti-LGR5 antibody and 20ul. (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
GPR49 / LGR5 Antibody - Figure from citation: Cytofluorimetric analysis of Lgr5 expression by PKH high cells over 4 weeks of culture. Data are expressed as percent mean values. (6 SD) of 5 consecutive experiments. Dilution: 1:400
GPR49 / LGR5 Antibody - HEK293T cells transfected with eitheroverexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-LGR5 antibody, and then analyzed by flow cytometry.
GPR49 / LGR5 Antibody - HEK293T cells transfected with eitheroverexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-LGR5 antibody, and then analyzed by flow cytometry.
GPR49 / LGR5 Antibody - Figure from citation: Flow cytometry of the SB cells. The G4 region was further divided into P1 and P2. Lgr5, a stem cell marker, was expressed by 32% of the P2 population. Black: staining with Lgr5; red: staining with the isotype control.
GPR49 / LGR5 Antibody - Figure from citation: Flow cytometry of the SB cells. The G4 region was further divided into P1 and P2. Lgr5, a stem cell marker, was expressed by 32% of the P2 population. Black: staining with Lgr5; red: staining with the isotype control.
GPR49 / LGR5 Antibody - Figure from citation: Cytofluorimetric analysis of Lgr5 expression by PKH high cells over 4 weeks of culture. Data are expressed as percent mean values. (6 SD) of 5 consecutive experiments. Dilution: 1:400
GPR49 / LGR5 Antibody - Figure from citation: Positive correlation between LSD1, c-Myc, ß-catenin and LGR5 expression in human colorectal tumor tissues. Expression levels of LSD1, LGR5, ß-catenin and c-Myc in consecutive sections from normal colon, adenoma and CRC tumor tissues. Dilution: 1:100
GPR49 / LGR5 Antibody - Figure from citation: Positive correlation between LSD1, c-Myc, ß-catenin and LGR5 expression in human colorectal tumor tissues. Expression levels of LSD1, LGR5, ß-catenin and c-Myc in consecutive sections from normal colon, adenoma and CRC tumor tissues. Dilution: 1:100
GPR49 / LGR5 Antibody - Figure from citation: Immunohistochemical staining of Lgr5 in normal gastric tissues. The normal gastric tissue, 40×magnified. (A) and 400× magnified. (B). Yellowspots represent the Lgr5 positive cells that are localized only at base of the glands in normal gastric mucosa. The arrows in the pictures show the immunoreactive cells stained in yellow color.
GPR49 / LGR5 Antibody - Figure from citation: Immunohistochemical staining of Lgr5 in normal gastric tissues. The normal gastric tissue, 40×magnified. (A) and 400× magnified. (B). Yellowspots represent the Lgr5 positive cells that are localized only at base of the glands in normal gastric mucosa. The arrows in the pictures show the immunoreactive cells stained in yellow color.
GPR49 / LGR5 Antibody - Figure from citation: Immunohistochemical analysis of Lgr5 expression in human normal colon biopsies; Lgr5+ cells are localized at the crypt base, where bona fide stem-like cells home; magnification is indicated in the boxes. Dilution: 1:200
GPR49 / LGR5 Antibody - Figure from citation: Immunohistochemical analysis of Lgr5 expression in human normal colon biopsies; Lgr5+ cells are localized at the crypt base, where bona fide stem-like cells home; magnification is indicated in the boxes. Dilution: 1:200
GPR49 / LGR5 Antibody - Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-LGR5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min,
GPR49 / LGR5 Antibody - Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-LGR5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min,
GPR49 / LGR5 Antibody - Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-LGR5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min,
GPR49 / LGR5 Antibody - Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-LGR5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min,
GPR49 / LGR5 Antibody - Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-LGR5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min,
GPR49 / LGR5 Antibody - Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-LGR5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min,
GPR49 / LGR5 Antibody - Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-LGR5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min,
GPR49 / LGR5 Antibody - Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-LGR5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min,
GPR49 / LGR5 Antibody - Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-LGR5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min,
GPR49 / LGR5 Antibody - Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-LGR5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min,
GPR49 / LGR5 Antibody - Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-LGR5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min,
GPR49 / LGR5 Antibody - Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-LGR5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min,
GPR49 / LGR5 Antibody - Figure from citation: [Nature] Cells expressing the stem cell marker Lgr5 was detected by immunostaining of hCECs in 2D culture. <br />Pubmed id: 27398792<br />Memo: Human; IF
GPR49 / LGR5 Antibody - Figure from citation: Immunocytochemistry of epithelium stem cell phenotype. Isolated dental epithelium following 5-day culture, overwhelmingly expressed Lgr5. Dilution: 1:100
GPR49 / LGR5 Antibody - Figure from citation: Immunocytochemistry of epithelium stem cell phenotype. Isolated dental epithelium following 5-day culture, overwhelmingly expressed Lgr5. Dilution: 1:100
GPR49 / LGR5 Antibody - Figure from citation: [Nature] Cells expressing the stem cell marker Lgr5 was detected by immunostaining of hCECs in 2D culture. <br />Pubmed id: 27398792<br />Memo: Human; IF
GPR49 / LGR5 Antibody - Anti-LGR5 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY LGR5.
GPR49 / LGR5 Antibody - Anti-LGR5 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY LGR5.
GPR49 / LGR5 Antibody - Immunofluorescent staining of HT29 cells using anti-LGR5 mouse monoclonal antibody green). Actin filaments were labeled with TRITC-phalloidin. (red), and nuclear with DAPI. (blue).
GPR49 / LGR5 Antibody - Immunofluorescent staining of HT29 cells using anti-LGR5 mouse monoclonal antibody green). Actin filaments were labeled with TRITC-phalloidin. (red), and nuclear with DAPI. (blue).
GPR49 / LGR5 Antibody - HEK293T cells were transfected with the pCMV6-ENTRY control. (Left lane) or pCMV6-ENTRY LGR5. (Right lane) cDNA for 48 hrs and lysed
GPR49 / LGR5 Antibody - HEK293T cells were transfected with the pCMV6-ENTRY control. (Left lane) or pCMV6-ENTRY LGR5. (Right lane) cDNA for 48 hrs and lysed
GPR49 / LGR5 Antibody - Immunoprecipitation(IP) of LGR5 by using TrueMab monoclonal anti-LGR5 antibodies. (Negative control: IP without adding anti-LGR5 antibody.). For each experiment, 500ul of DDK tagged LGR5 overexpression lysates. (at 1:5 dilution with HEK293T lysate), 2ug of anti-LGR5 antibody and 20ul. (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
GPR49 / LGR5 Antibody - Immunoprecipitation(IP) of LGR5 by using TrueMab monoclonal anti-LGR5 antibodies. (Negative control: IP without adding anti-LGR5 antibody.). For each experiment, 500ul of DDK tagged LGR5 overexpression lysates. (at 1:5 dilution with HEK293T lysate), 2ug of anti-LGR5 antibody and 20ul. (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
GPR49 / LGR5 Antibody - Figure from citation: Cytofluorimetric analysis of Lgr5 expression by PKH high cells over 4 weeks of culture. Data are expressed as percent mean values. (6 SD) of 5 consecutive experiments. Dilution: 1:400
GPR49 / LGR5 Antibody - HEK293T cells transfected with eitheroverexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-LGR5 antibody, and then analyzed by flow cytometry.
GPR49 / LGR5 Antibody - HEK293T cells transfected with eitheroverexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-LGR5 antibody, and then analyzed by flow cytometry.
GPR49 / LGR5 Antibody - Figure from citation: Flow cytometry of the SB cells. The G4 region was further divided into P1 and P2. Lgr5, a stem cell marker, was expressed by 32% of the P2 population. Black: staining with Lgr5; red: staining with the isotype control.
GPR49 / LGR5 Antibody - Figure from citation: Flow cytometry of the SB cells. The G4 region was further divided into P1 and P2. Lgr5, a stem cell marker, was expressed by 32% of the P2 population. Black: staining with Lgr5; red: staining with the isotype control.
GPR49 / LGR5 Antibody - Figure from citation: Cytofluorimetric analysis of Lgr5 expression by PKH high cells over 4 weeks of culture. Data are expressed as percent mean values. (6 SD) of 5 consecutive experiments. Dilution: 1:400
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Monoclonal Mouse anti‑Human GPR49 / LGR5 Antibody (clone OTI2A2, Carrier‑free, aa250‑550, IHC, IF, WB) LS‑C797459

Monoclonal Mouse anti‑Human GPR49 / LGR5 Antibody (clone OTI2A2, Carrier‑free, aa250‑550, IHC, IF, WB) LS‑C797459

Submit Review LSBio Guarantee
Antibody:
GPR49 / LGR5 Mouse anti-Human Monoclonal (aa250-550) (Carrier-free) (OTI2A2) Antibody
Application:
IHC, IF, WB, IP, Flo
Reactivity:
Human, Mouse
Format:
Unconjugated, Carrier-free
Other formats:
Unmodified
Price
Catalog Number
$511
LS-C797459-100
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
GPR49 / LGR5 Mouse anti-Human Monoclonal (aa250-550) (Carrier-free) (OTI2A2) Antibody
Application:
IHC, IF, WB, IP, Flo
Reactivity:
Human, Mouse
Format:
Unconjugated, Carrier-free
Other formats:
Unmodified

Specifications

Description
LGR5 antibody LS-C797459 is an unconjugated mouse monoclonal antibody to LGR5 (GPR49) (aa250-550) from human. It is reactive with human and mouse. Validated for Flow, IF, IHC, IP and WB.
Target
Human GPR49 / LGR5
Synonyms
LGR5 | FEX | G-protein coupled receptor 49 | G protein-coupled receptor 49 | GPR67 | GRP49 | GPR49 | HG38 | G-protein coupled receptor 67
Host
Mouse
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Clone
OTI2A2
Conjugations
Unconjugated
Purification
Purified from ascites.
Modifications
Carrier-free. Also available Unmodified.
Immunogen
Human recombinant protein fragment corresponding to amino acids 250-550 of human LGR5 (NP_003658) produced in HEK293T Cell.
Epitope
aa250-550
Applications
  • IHC (1:150)
  • Immunofluorescence (1:100)
  • Western blot (1:2000)
  • Immunoprecipitation (4 µg/ml)
  • Flow Cytometry (1:100)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Applications should be user optimized.
Presentation
PBS, pH 7.3, 8% Trehalose
Reconstitution
Reconstitute with PBS pH 7.3. To use this carrier-free antibody for conjugation experiment, we strongly recommend you to perform another round of desalting process.
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About GPR49 / LGR5
O75473 NM_003667 NP_003658.1

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To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/28/2024
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