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Antibodies > ER Alpha / Estrogen Receptor > LS-C157726
 
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Catalog Number Size Price
LS-C157726-400 400 µl $393 
ER Alpha / Estrogen Receptor Antibody - Fluorescent confocal image of MCF-7 cell stained with ESR1 isoform1 Antibody. MCF-7 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ESR1 isoform1 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). ESR1 isoform1 immunoreactivity is localized to cytoplasm significantly.
ER Alpha / Estrogen Receptor Antibody - Western blot of ESR1 isoform1 Antibody antibody pre-incubated without(lane 1) and with(lane 2) blocking peptide in K562 cell line lysate. ESR1 isoform1 Antibody (arrow) was detected using the purified antibody.
ER Alpha / Estrogen Receptor Antibody - Fluorescent confocal image of MCF-7 cell stained with ESR1 isoform1 Antibody. MCF-7 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ESR1 isoform1 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). ESR1 isoform1 immunoreactivity is localized to cytoplasm significantly.
ER Alpha / Estrogen Receptor Antibody - Western blot of ESR1 isoform1 Antibody antibody pre-incubated without(lane 1) and with(lane 2) blocking peptide in K562 cell line lysate. ESR1 isoform1 Antibody (arrow) was detected using the purified antibody.
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Polyclonal Rabbit anti‑Human ER Alpha / Estrogen Receptor Antibody (aa452‑480, IF, WB) LS‑C157726

Polyclonal Rabbit anti‑Human ER Alpha / Estrogen Receptor Antibody (aa452‑480, IF, WB) LS‑C157726

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Antibody:
ER Alpha / Estrogen Receptor Rabbit anti-Human Polyclonal (aa452-480) Antibody
Application:
IF, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$393
LS-C157726-400
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
ER Alpha / Estrogen Receptor Rabbit anti-Human Polyclonal (aa452-480) Antibody
Application:
IF, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
Estrogen Receptor antibody LS-C157726 is an unconjugated rabbit polyclonal antibody to human Estrogen Receptor (ER Alpha) (aa452-480). Validated for IF and WB.
Target
Human ER Alpha / Estrogen Receptor
Synonyms
ESR1 | Era | ERalpha | Estrogen receptor | ER-alpha | Estrogen receptor 1 | NR3A1 | ER | ESR | ESRA | Estrogen receptor alpha
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Mouse, Rat, Pig (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Epitope
aa452-480
Specificity
This ESR1 isoform1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 452-480 amino acids from the C-terminal region of human ESR1 isoform1.
Applications
  • Immunofluorescence (1:10 - 1:50)
  • Western blot (1:1000)
Presentation
PBS, 0.09% Sodium Azide
Storage
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ER Alpha / Estrogen Receptor
P03372 NM_000125 NP_000116.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 5/2/2024
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