Human COL3A1 / Collagen III

Rabbit

Human, Pig, Cow (tested or 100% immunogen sequence identity)

Polyclonal

Unconjugated

Unmodified

Procollagen type III deprived of the C-Terminus PIIICP peptide

• IHC - Paraffin (1:500)
• Immunofluorescence (1:40)
• Western blot
• ELISA (1:100 - 1:200)
• Radioimmunoassay (>1:200)

After de-waxing the tissue slices they are treated with 0.2% hyaluronidase (app. 300 U/mg) in TBS 15 minutes at 37°C. Thereafter non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 minutes at RT is recommended. Collagen type III is synthesized as a homo-trimeric pro-collagen comprising three identical pro-alpha(III)-chains. It has been reported that procollagen type III is processed extracellularly at the ECM and can be found by immunostaining intracellular as well as extracellular. Collagens consist of a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule consists of a triple-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs. Human and bovine procollagen type III (PIIIP) 100%; human and bovine procollagen type and collagen type I <0.1% in RIA at 1:200 dilution..

PBS

0.1 ml Reconstitute in sterile distilled water.

Store at -20°C.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P02461 NCBI: NM_000090 NP_000081.1