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Catalog Number Size Price
LS-C662081-10 10 µg $318 
LS-C662081-100 100 µg $470 
CHI3L1 / YKL-40 Antibody - Western blot analysis of CHI3L1 using anti-CHI3L1 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse RAW264. 7 whole cell lysates,Lane 2: mouse HEPA1-6 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CHI3L1 antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4?, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CHI3L1 at approximately 43KD. The expected band size for CHI3L1 is at 43KD.
CHI3L1 / YKL-40 Antibody - Western blot - Anti-CHI3L1/Gp 39 Picoband Antibody
CHI3L1 / YKL-40 Antibody - Western blot analysis of CHI3L1 using anti-CHI3L1 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse RAW264. 7 whole cell lysates,Lane 2: mouse HEPA1-6 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CHI3L1 antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4?, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CHI3L1 at approximately 43KD. The expected band size for CHI3L1 is at 43KD.
CHI3L1 / YKL-40 Antibody - Western blot - Anti-CHI3L1/Gp 39 Picoband Antibody
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Polyclonal Rabbit anti‑Mouse CHI3L1 / YKL‑40 Antibody (WB) LS‑C662081

Polyclonal Rabbit anti‑Mouse CHI3L1 / YKL‑40 Antibody (WB) LS‑C662081

Antibody:
CHI3L1 / YKL-40 Rabbit anti-Mouse Polyclonal Antibody
Application:
WB, ELISA
Reactivity:
Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C662081-10
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
CHI3L1 / YKL-40 Rabbit anti-Mouse Polyclonal Antibody
Application:
WB, ELISA
Reactivity:
Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
YKL-40 antibody LS-C662081 is an unconjugated rabbit polyclonal antibody to mouse YKL-40 (CHI3L1). Validated for ELISA and WB.
Target
Mouse CHI3L1 / YKL-40
Synonyms
CHI3L1 | 39 kDa synovial protein | ASRT7 | Cartilage glycoprotein 39 | CGP-39 | Chitinase-3-like protein 1 | gp39 | HCGP-39 | HCGP-3P | GP-39 | HC-gp39 | YKL40 | YYL-40 | YKL-40
Host
Rabbit
Reactivity
Mouse (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
E. coli-derived mouse CHI3L1 recombinant protein (Position: Y30-K262).
Specificity
Detected in lung in pulmonary macrophages and alveolar type 2 cells and in bronchoalveolar lavage (BAL) fluids. Expressed in mammary tumor cells (at protein level). Expressed in lung. Not detected in non-inflammatory colon.
Applications
  • Western blot
  • ELISA
Presentation
Lyophilized from 0.2mg Na2HPO4, 0.9mg NaCl, 0.05mg sodium azide, 4mg Trehalose
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CHI3L1 / YKL-40
P36222 NM_001276 NP_001267.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/27/2024