Target
Mouse TNF Alpha
Synonyms
TNF | APC1 protein | Cachectin | DIF | TNF Alpha | Tumor necrosis factor-alpha | TNF-alpha | TNF-a | TNFA | Tumor necrosis factor | TNF, macrophage-derived | TNF, monocyte-derived | TNFSF2
Reactivity
Mouse
(tested or 100% immunogen sequence identity)
Clonality
IgG1
Monoclonal
Conjugations
Unconjugated
Purification
Ion exchange chromatography
Immunogen
Recombinant mouse TNFa expressed in E. coli
Specificity
Binds and neutralizes murine TNFa. Does not recognize human or rat TNFa.
Applications
- Western blot (1 µg/ml)
- Neutralization (3 µg/ml)
Usage
Suitable for use in Dot Blot and Neutralization. Dot Blot: 1 ug/ml detected 5 ng recombinant murine TNFa. Neutralization: 3 ug/ml neutralized 50% of the cytotoxic effect of 50pg/ml recombinant mouse TNFa on actinomycin D treated L929 cells. The antibody concentration needed to neutralize the bioactivity of TNFa will depend on cell type, incubation conditions and biological response.
Presentation
0.01 M Tris-glycine, pH 7.4, 0.15 M sodium chloride.
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee