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Anti-Sprouty 4 / SPRY4 Antibody (aa306-322) IHC-plus™ LS-B77

Ordering

Wt. Vol. Conc. Price
50 µg - 1 mg/ml $395

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Sprouty 4 / SPRY4 Antibody LS-B77 Images

[click image for a larger image and more details]
Immunohistochemistry
Anti-SPRY4 antibody IHC of human heart.
Western Blot
Anti-Sprouty-4 Antibody - Western Blot.

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Rabbit Polyclonal to Human Sprouty 4 / SPRY4
Human, Chimpanzee
IHC - Paraffin, Western blot, ELISA
Unconjugated

Details

Human Sprouty 4 / SPRY4
Rabbit
Human, Chimpanzee (tested or 100% immunogen sequence identity)
Polyclonal
Immunoaffinity purified

Applications

IHC - Paraffin (2.5 µg/ml), Western blot (1:100 - 1:1000), ELISA (1:3000 - 1:12000)

Specificity and Use

Sprouty 4 / SPRY4 antibody was raised against synthetic peptide from human SPRY4.
aa306-322
Amino acids 306-322 of Human Sprouty-4 protein.
Immunohistochemistry: LS-B77 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B77 was determined to be 2.5 ug/ml.

Packaging

0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
+4°C or -20°C, Avoid repeated freezing and thawing.
For research use only.

About Sprouty 4 / SPRY4

Q9C004 NM_030964 NP_112226.2

SPRY4 Antibody, Protein sprouty homolog 4 Antibody, Sprouty 4 Antibody, Sprouty homolog 4 (Drosophila) Antibody, Sprouty 4 Antibody, Spry-4 Antibody

Sprouty-4 (also known as SPRY4) is an inhibitor of the insulin receptor and EGFR-transduced mitogen-activated protein kinase (MAPK) signaling pathway. It is positioned upstream of RAS activation and impairs the formation of active GTP-RAS. Sprouty-4 is a cytoplasmic protein that interacts with the C-terminus of TESK1. Multiple splice variants have been reported for this protein.


Sprouty 4 / SPRY4 Antibodies (26)

Target Reactivity Applications Host Clonality Clone Format Epitope Publications
Sprouty 4 / SPRY4 Rabbit Polyclonal Unconjugated Human IHC-P, WB C-Terminus No
Anti-Sprouty 4 / SPRY4 Antibody (C-Terminus) IHC-plus™ LS-B9112
IHC Image
WB Image
Rabbit Polyclonal to Human Sprouty 4 / SPRY4
Human
IHC - Paraffin, Western blot
Unconjugated
Sprouty 4 / SPRY4 Rabbit Polyclonal Unconjugated Human WB Internal No
Anti-Sprouty 4 / SPRY4 Antibody (Internal) LS-C116397
WB Image
Rabbit Polyclonal to Human Sprouty 4 / SPRY4
Human
Western blot
Unconjugated
Sprouty 4 / SPRY4 Rabbit Polyclonal Unconjugated Human IHC-P, WB, Flo aa57-86 No
Anti-Sprouty 4 / SPRY4 Antibody (aa57-86) LS-C161477
IHC Image
Flo Image
Rabbit Polyclonal to Human Sprouty 4 / SPRY4
Human
IHC - Paraffin, Western blot, Flow Cytometry
Unconjugated
Sprouty 4 / SPRY4 Rabbit Polyclonal Unconjugated Human IHC, WB, ELISA N-Terminus No
Anti-Sprouty 4 / SPRY4 Antibody (N-Terminus) LS-C153564
Rabbit Polyclonal to Human Sprouty 4 / SPRY4
Human
IHC, Western blot, ELISA
Unconjugated
Sprouty 4 / SPRY4 Rabbit Polyclonal Unconjugated Human IHC, WB N-Terminus No
Anti-Sprouty 4 / SPRY4 Antibody (N-Terminus) LS-C144695
Rabbit Polyclonal to Human Sprouty 4 / SPRY4
Human
IHC, Western blot
Unconjugated
Sprouty 4 / SPRY4 Rabbit Polyclonal FITC Conjugated Human IHC, WB, Flo, ELISA aa57-86 No
Anti-Sprouty 4 / SPRY4 Antibody (aa57-86) (FITC) LS-C224140
Rabbit Polyclonal (IgG) to Human Sprouty 4 / SPRY4
Human
IHC, Western blot, Flow Cytometry, ELISA
FITC Conjugated
Sprouty 4 / SPRY4 Rabbit Polyclonal HRP Conjugated Human IHC, WB, Flo, ELISA aa57-86 No
Anti-Sprouty 4 / SPRY4 Antibody (aa57-86) (HRP) LS-C224141
Rabbit Polyclonal (IgG) to Human Sprouty 4 / SPRY4
Human
IHC, Western blot, Flow Cytometry, ELISA
HRP Conjugated
Sprouty 4 / SPRY4 Rabbit Polyclonal PE Conjugated Human IHC, WB, Flo, ELISA aa57-86 No
Anti-Sprouty 4 / SPRY4 Antibody (aa57-86) (PE) LS-C224143
Rabbit Polyclonal (IgG) to Human Sprouty 4 / SPRY4
Human
IHC, Western blot, Flow Cytometry, ELISA
PE Conjugated
Sprouty 4 / SPRY4 Rabbit Polyclonal AP Conjugated Human IHC, WB, Flo, ELISA aa57-86 No
Anti-Sprouty 4 / SPRY4 Antibody (aa57-86) (AP) LS-C224137
Rabbit Polyclonal (IgG) to Human Sprouty 4 / SPRY4
Human
IHC, Western blot, Flow Cytometry, ELISA
AP Conjugated
Sprouty 4 / SPRY4 Rabbit Polyclonal APC Conjugated Human IHC, WB, Flo, ELISA aa57-86 No
Anti-Sprouty 4 / SPRY4 Antibody (aa57-86) (APC) LS-C224138
Rabbit Polyclonal (IgG) to Human Sprouty 4 / SPRY4
Human
IHC, Western blot, Flow Cytometry, ELISA
APC Conjugated
Sprouty 4 / SPRY4 Rabbit Polyclonal Biotin Conjugated Human IHC, WB, Flo, ELISA aa57-86 No
Anti-Sprouty 4 / SPRY4 Antibody (aa57-86) (Biotin) LS-C224139
Rabbit Polyclonal (IgG) to Human Sprouty 4 / SPRY4
Human
IHC, Western blot, Flow Cytometry, ELISA
Biotin Conjugated
Sprouty 4 / SPRY4 Rabbit Polyclonal Unconjugated Human, Gorilla, Gibbon, Monkey IHC, WB, ELISA aa283-299 No
Anti-Sprouty 4 / SPRY4 Antibody (aa283-299) LS-C89244
Rabbit Polyclonal (IgG) to Human Sprouty 4 / SPRY4
Human, Gorilla, Gibbon, Monkey
IHC, Western blot, ELISA
Unconjugated
Sprouty 4 / SPRY4 Rabbit Polyclonal Unconjugated Human DB pTyr75 No
Anti-Sprouty 4 / SPRY4 Antibody (phospho-Tyr75) LS-C97105
DB Image
Rabbit Polyclonal to Human Sprouty 4 / SPRY4
Human
Dot Blot
Unconjugated
Sprouty 4 / SPRY4 Rabbit Polyclonal Biotin Conjugated Human WB, ELISA C-Terminus No
Anti-Sprouty 4 / SPRY4 Antibody (C-Terminus) (Biotin) LS-C226405
Rabbit Polyclonal (IgG) to Human Sprouty 4 / SPRY4
Human
Western blot, ELISA
Biotin Conjugated
Sprouty 4 / SPRY4 Rabbit Polyclonal APC Conjugated Human WB, ELISA C-Terminus No
Anti-Sprouty 4 / SPRY4 Antibody (C-Terminus) (APC) LS-C226404
Rabbit Polyclonal (IgG) to Human Sprouty 4 / SPRY4
Human
Western blot, ELISA
APC Conjugated
Sprouty 4 / SPRY4 Rabbit Polyclonal AP Conjugated Human WB, ELISA C-Terminus No
Anti-Sprouty 4 / SPRY4 Antibody (C-Terminus) (AP) LS-C226403
Rabbit Polyclonal (IgG) to Human Sprouty 4 / SPRY4
Human
Western blot, ELISA
AP Conjugated
Sprouty 4 / SPRY4 Rabbit Polyclonal PE Conjugated Human WB, ELISA C-Terminus No
Anti-Sprouty 4 / SPRY4 Antibody (C-Terminus) (PE) LS-C226409
Rabbit Polyclonal (IgG) to Human Sprouty 4 / SPRY4
Human
Western blot, ELISA
PE Conjugated
Sprouty 4 / SPRY4 Rabbit Polyclonal FITC Conjugated Human WB, ELISA C-Terminus No
Anti-Sprouty 4 / SPRY4 Antibody (C-Terminus) (FITC) LS-C226407
Rabbit Polyclonal (IgG) to Human Sprouty 4 / SPRY4
Human
Western blot, ELISA
FITC Conjugated
Sprouty 4 / SPRY4 Rabbit Polyclonal HRP Conjugated Human WB, ELISA C-Terminus No
Anti-Sprouty 4 / SPRY4 Antibody (C-Terminus) (HRP) LS-C226406
Rabbit Polyclonal (IgG) to Human Sprouty 4 / SPRY4
Human
Western blot, ELISA
HRP Conjugated
Sprouty 4 / SPRY4 Rabbit Polyclonal Biotin Conjugated Human ELISA, DB pTyr75 No
Anti-Sprouty 4 / SPRY4 Antibody (phospho-Tyr75) (Biotin) LS-C226399
Rabbit Polyclonal (IgG) to Human Sprouty 4 / SPRY4
Human
ELISA, Dot Blot
Biotin Conjugated
Sprouty 4 / SPRY4 Rabbit Polyclonal APC Conjugated Human ELISA, DB pTyr75 No
Anti-Sprouty 4 / SPRY4 Antibody (phospho-Tyr75) (APC) LS-C226398
Rabbit Polyclonal (IgG) to Human Sprouty 4 / SPRY4
Human
ELISA, Dot Blot
APC Conjugated
Sprouty 4 / SPRY4 Rabbit Polyclonal AP Conjugated Human ELISA, DB pTyr75 No
Anti-Sprouty 4 / SPRY4 Antibody (phospho-Tyr75) (AP) LS-C226397
Rabbit Polyclonal (IgG) to Human Sprouty 4 / SPRY4
Human
ELISA, Dot Blot
AP Conjugated
Sprouty 4 / SPRY4 Rabbit Polyclonal PE Conjugated Human ELISA, DB pTyr75 No
Anti-Sprouty 4 / SPRY4 Antibody (phospho-Tyr75) (PE) LS-C226402
Rabbit Polyclonal (IgG) to Human Sprouty 4 / SPRY4
Human
ELISA, Dot Blot
PE Conjugated
Sprouty 4 / SPRY4 Rabbit Polyclonal HRP Conjugated Human ELISA, DB pTyr75 No
Anti-Sprouty 4 / SPRY4 Antibody (phospho-Tyr75) (HRP) LS-C226401
Rabbit Polyclonal (IgG) to Human Sprouty 4 / SPRY4
Human
ELISA, Dot Blot
HRP Conjugated
Sprouty 4 / SPRY4 Rabbit Polyclonal FITC Conjugated Human ELISA, DB pTyr75 No
Anti-Sprouty 4 / SPRY4 Antibody (phospho-Tyr75) (FITC) LS-C226400
Rabbit Polyclonal (IgG) to Human Sprouty 4 / SPRY4
Human
ELISA, Dot Blot
FITC Conjugated
Sprouty 4 / SPRY4 Rabbit Polyclonal Unconjugated Human, Chimpanzee IHC-P, WB, ELISA aa306-322 No
Anti-Sprouty 4 / SPRY4 Antibody (aa306-322) IHC-plus™ LS-B77
IHC Image
WB Image
Rabbit Polyclonal to Human Sprouty 4 / SPRY4
Human, Chimpanzee
IHC - Paraffin, Western blot, ELISA
Unconjugated

Immunohistochemistry

Immunohistochemistry
Anti-SPRY4 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B77 concentration 5 ug/ml.

Western Blot

Western Blot
Anti-Sprouty-4 Antibody - Western Blot. Western blot of Affinity Purified anti-Sprouty-4 antibody shows detection of a doublet band ~35 kD corresponding to human Sprouty-4. Approximately 30 ug of HeLa (lane 1), SW13 (lane 2) and C2C12 (Lane 3) whole cell lysates were separated by 10% SDS-PAGE and transferred onto nitrocellulose. After blocking with 5% non-fat dry milk in TBST the membrane was probed with the primary antibody diluted to 1:100 overnight at 4C followed by washes and reaction with a 1:5000 dilution of HRP conjugated Gt-a-Rabbit IgG [H&L] MX (LS-C60865) for 1 h at room temperature. Signal was procebetaed by ECL (Pierce). Other detection systems will yield similar results.

Requested From: United States
Date Requested: 4/18/2014
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