Human, Chimpanzee (tested or 100% immunogen sequence identity)
IHC - Paraffin (2.5 µg/ml), Western blot (1:100 - 1:1000), ELISA (1:3000 - 1:12000)
Specificity and Use
Sprouty 4 / SPRY4 antibody was raised against synthetic peptide from human SPRY4.
Amino acids 306-322 of Human Sprouty-4 protein.
Immunohistochemistry: LS-B77 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B77 was determined to be 2.5 ug/ml.
0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
+4°C or -20°C, Avoid repeated freezing and thawing.
Sprouty-4 (also known as SPRY4) is an inhibitor of the insulin receptor and EGFR-transduced mitogen-activated protein kinase (MAPK) signaling pathway. It is positioned upstream of RAS activation and impairs the formation of active GTP-RAS. Sprouty-4 is a cytoplasmic protein that interacts with the C-terminus of TESK1. Multiple splice variants have been reported for this protein.
Anti-SPRY4 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B77 concentration 5 ug/ml.
Anti-Sprouty-4 Antibody - Western Blot. Western blot of Affinity Purified anti-Sprouty-4 antibody shows detection of a doublet band ~35 kD corresponding to human Sprouty-4. Approximately 30 ug of HeLa (lane 1), SW13 (lane 2) and C2C12 (Lane 3) whole cell lysates were separated by 10% SDS-PAGE and transferred onto nitrocellulose. After blocking with 5% non-fat dry milk in TBST the membrane was probed with the primary antibody diluted to 1:100 overnight at 4C followed by washes and reaction with a 1:5000 dilution of HRP conjugated Gt-a-Rabbit IgG [H&L] MX (LS-C60865) for 1 h at room temperature. Signal was procebetaed by ECL (Pierce). Other detection systems will yield similar results.
Requested From: United States
Date Requested: 4/18/2014