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Catalog Number Size Price
LS-C209429-100 100 µg (0.94 mg/ml) $567 
SMAD2 Antibody - Western Blot of Rabbit anti-Smad2 pS465pS467 antibody. Lane 1: MDA-MB-231 cells. Lane 2: MDA-MB-231 cells treated with TGF-ß1 for 1h. Load: 20 µg per lane. Primary antibody: Smad2pS465pS467 antibody at 1:1000 for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10,000 for 45 min at RT.

Polyclonal Rabbit anti‑Human SMAD2 Antibody (phospho‑Ser465/467, WB) LS‑C209429

Polyclonal Rabbit anti‑Human SMAD2 Antibody (phospho‑Ser465/467, WB) LS‑C209429

Antibody:
SMAD2 Rabbit anti-Human Polyclonal (pSer465/467) Antibody
Application:
WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$567
LS-C209429-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
SMAD2 Rabbit anti-Human Polyclonal (pSer465/467) Antibody
Application:
WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
SMAD2 antibody LS-C209429 is an unconjugated rabbit polyclonal antibody to human SMAD2 (pSer465/467). Validated for ELISA and WB.
Target
Human SMAD2
Synonyms
SMAD2 | HMAD-2 | HSMAD2 | JV18-1 | Mad protein homolog smad2 | MADR2 | MADH2 | SMAD 2 | MAD homolog 2 | Mad-related protein 2 | JV18 | Mother against DPP homolog 2 | Mothers against DPP homolog 2 | Sma- and Mad-related protein 2 | SMAD family member 2
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Orangutan, Mouse, Rat, Bovine (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
SMAD2 pS465 pS467 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a C-terminal of human SMAD2 protein.
Epitope
pSer465/467
Specificity
Anti-SMAD2 pS465 pS467 antibody is directed against the phosphorylated form of human Smad2 protein at the pS465 and pS467 residues. Reactivity occurs against human SMAD2. Reactivity with non-phosphorylated human Smad2 is minimal by ELISA and western blot. A BLAST analysis was used to suggest cross-reactivity with Smad2 protein from human, mouse, rat, orangutan, and bovine based on 100% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.
Applications
  • Western blot (1:1000 - 1:3000)
  • ELISA (1:10000)
Usage
Anti-SMAD2 pS465 pS467 affinity purified antibody has been tested for use in ELISA and western blotting. Expect a band approximately 52 kD in size corresponding to Smad2 protein by western blotting in the appropriate cell lysate or extract.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Centrifuge product before removing cap. Only dilute immediately prior to use.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About SMAD2
Q15796 NM_005901 NP_005892.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/26/2024