Human, Gorilla, Gibbon (tested or 100% immunogen sequence identity)
Monkey (at least 90% immunogen sequence identity)
IHC - Paraffin (3.75 µg/ml)
Specificity and Use
SLC46A1 / HCP1 antibody was raised against synthetic peptide YNGTRQRGGCSNRS from an internal region of human SLC46A1 / HCP1 (NP_542400.2). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%); Marmoset (93%).
Immunohistochemistry: LS-B3827 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3827 was determined to be 3.75ug/ml.