Human, Macaque (tested or 100% immunogen sequence identity)
IHC - Paraffin (2.5 µg/ml), Western blot (1:1000 - 1:3000), ELISA (1:3200 - 1:10000)
Specificity and Use
PPARG / PPAR Gamma antibody was raised against synthetic peptide from human PPARG.
A region near the amino terminus of human PPAR gamma 2.
Immunohistochemistry: LS-B130 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B130 was determined to be 2.5 ug/ml.
0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
Peroxisome proliferator-activated receptor gamma (PPAR gamma), a NR1 Thyroid Hormone-Like Receptor, is a transcription factor that regulates genes involved in lipid metabolism and adipocyte differentiation and has been shown to affect placental differentiation and cell proliferation and differentiation pathways in various diseases such as Type II diabetes and atherosclerosis. Recently, PPAR gamma has been suggested to affect inflammatory digestive diseases (Dubuquoy et al. 2002).
Anti-PPARG antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B130 concentration 5 ug/ml.
Anti-PPAR2 Antibody - Western Blot. Western blot of affinity purified anti-PPAR2 antibody shows detection of PPAR2 protein in a mouse 3T3 whole cell lysate (lane 1 arrowhead). Approximately 20 ug of lysate was loaded onto a 4-20% gradient gel followed by transfer to nitrocellulose. Primary antibody was used at a 1:2000 dilution in 5% BLOTTO PBS solution. The membrane was washed and reacted with a 1:10000 dilution of IRDye800 Conjugated Affinity Purified Goat-anti-Rabbit IgG [H&L] MX10. Molecular weight estimation was made by comparison to prestained MW markers indicated at the left (lane M). Other detection systems will yield similar results.
Requested From: United States
Date Requested: 3/7/2014