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Anti-PLK1 / PLK-1 Antibody (phospho-Thr210) [clone 2A3] IHC-plus™ LS-B1675

Ordering

Wt. Vol. Conc. Price
50 µg - 0.5 mg/ml $395

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

PLK1 / PLK-1 Antibody LS-B1675 Images

[click image for a larger image and more details]
Immunohistochemistry
Anti-PLK1 antibody IHC of human colon.
Western Blot
Panel A.
Western Blot
 

100% Guaranteed 100% Guaranteed Publications Publications (1)
Mouse Monoclonal [clone 2A3] (IgG1) to Human PLK1 / PLK-1
Human
IHC - Paraffin, Western blot
Unconjugated

Details

Human PLK1 / PLK-1
Mouse
Human (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [2A3]
Affinity purified

Applications

IHC - Paraffin (15 µg/ml), Western blot

Specificity and Use

PLK1 / PLK-1 antibody was raised against synthetic peptide from human PLK1.
pThr210
Modified peptide
Immunohistochemistry: LS-B1675 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1675 was determined to be 15 ug/ml.

Packaging

Phosphate-buffered solution, pH 7.2, 0.09% sodium azide at 0.5 mg/ml. Sourced in TCS.
+4°C, avoid freezing
For research use only.

Publications (1)

1
Arpc1b, a centrosomal protein, is both an activator and substrate of Aurora A. Molli PR, Li DQ, Bagheri-Yarmand R, Pakala SB, Katayama H, Sen S, Iyer J, Chernoff J, Tsai MY, Nair SS, Kumar R. The Journal of cell biology. 2010 190:101-14. [PubMed:20603326] [PMC:PMC2911675]

About PLK1 / PLK-1

P53350 NM_005030 NP_005021.2

PLK1 Antibody, Polo like kinase Antibody, Polo-like kinase (Drosophila) Antibody, Polo-like kinase 1 Antibody, STPK13 Antibody, Polo (Drosophia)-like kinase Antibody, PLK Antibody, PLK-1 Antibody

PLK, a Polo type protein kinase, has been shown to function in mitosis. The mRNA is absent or expressed at very low levels during the G1 phase, begins to reaccumulate during the S phase, and reaches maximal levels during the G2/M phase. PLK1 may participate in targeting MPF to the nucleus during prophase and in regulating mitotic spindle function. These functions, in addition to its expression in proliferating cells, suggest that PLK1 has a role in cancer progression.


PLK1 / PLK-1 Antibodies (53)

Target Reactivity Applications Host Clonality Clone Format Epitope Publications
PLK1 / PLK-1 Rabbit Polyclonal Unconjugated Human IHC-P, WB, ELISA pThr210 No
Anti-PLK1 / PLK-1 Antibody (phospho-Thr210) IHC-plus™ LS-B50
IHC Image
WB Image
Rabbit Polyclonal to Human PLK1 / PLK-1
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated
PLK1 / PLK-1 Rabbit Polyclonal Unconjugated Human IHC-P, WB, IP No
Anti-PLK1 / PLK-1 Antibody IHC-plus™ LS-B1674
IHC Image
WB Image
Rabbit Polyclonal (IgG) to Human PLK1 / PLK-1
Human
IHC - Paraffin, Western blot, Immunoprecipitation
Unconjugated
PLK1 / PLK-1 Rabbit Polyclonal Unconjugated Human IHC-P, WB No
Anti-PLK1 / PLK-1 Antibody IHC-plus™ LS-B4225
IHC Image
IHC Image
Rabbit Polyclonal to Human PLK1 / PLK-1
Human
IHC - Paraffin, Western blot
Unconjugated
PLK1 / PLK-1 Rabbit Polyclonal Unconjugated Human IHC-P, WB No
Anti-PLK1 / PLK-1 Antibody IHC-plus™ LS-B6646
IHC Image
WB Image
Rabbit Polyclonal (IgG) to Human PLK1 / PLK-1
Human
IHC - Paraffin, Western blot
Unconjugated
PLK1 / PLK-1 Rabbit Polyclonal Unconjugated Human, Monkey, Mouse, Rat, Bovine, Dog, Pig, Macaque IHC-P, WB aa100-200 No
Anti-PLK1 / PLK-1 Antibody (aa100-200) IHC-plus™ LS-B6508
IHC Image
WB Image
Rabbit Polyclonal to Human PLK1 / PLK-1
Human, Monkey, Mouse, Rat, Bovine, Dog, Pig, Macaque
IHC - Paraffin, Western blot
Unconjugated
PLK1 / PLK-1 Rabbit Polyclonal Unconjugated Human, Mouse IHC-P, WB C-Terminus No
Anti-PLK1 / PLK-1 Antibody (C-Terminus) IHC-plus™ LS-B5792
IHC Image
WB Image
Rabbit Polyclonal (IgG) to Human PLK1 / PLK-1
Human, Mouse
IHC - Paraffin, Western blot
Unconjugated
PLK1 / PLK-1 Mouse Monoclonal 6C6 Unconjugated Human, Dog IHC-P, WB, IP No
Anti-PLK1 / PLK-1 Antibody [clone 6C6] LS-C114507
IHC Image
IHC Image
Mouse Monoclonal [clone 6C6] (IgG2b) to Human PLK1 / PLK-1
Human, Dog
IHC - Paraffin, Western blot, Immunoprecipitation
Unconjugated
PLK1 / PLK-1 Mouse Monoclonal 1D4 Unconjugated Human IHC-P, WB, IP No
Anti-PLK1 / PLK-1 Antibody [clone 1D4] LS-C114495
IHC Image
IHC Image
Mouse Monoclonal [clone 1D4] (IgG2b) to Human PLK1 / PLK-1
Human
IHC - Paraffin, Western blot, Immunoprecipitation
Unconjugated
PLK1 / PLK-1 Mouse Monoclonal 5C1 Unconjugated Human IHC-P, WB, IP, Flo No
Anti-PLK1 / PLK-1 Antibody [clone 5C1] LS-C114502
IHC Image
IHC Image
Mouse Monoclonal [clone 5C1] (IgG2b) to Human PLK1 / PLK-1
Human
IHC - Paraffin, Western blot, Immunoprecipitation, Flow Cytometry
Unconjugated
PLK1 / PLK-1 Mouse Monoclonal 3C11 Unconjugated Human IHC, WB, Flo, ELISA No
Anti-PLK1 / PLK-1 Antibody [clone 3C11] LS-C169110
IHC Image
IHC Image
Mouse Monoclonal [clone 3C11] (IgG1) to Human PLK1 / PLK-1
Human
IHC, Western blot, Flow Cytometry, ELISA
Unconjugated
PLK1 / PLK-1 Mouse Monoclonal 3F12 Unconjugated Human IHC-P, WB, IP, Flo No
Anti-PLK1 / PLK-1 Antibody [clone 3F12] LS-C114491
IHC Image
Flo Image
Mouse Monoclonal [clone 3F12] (IgG1) to Human PLK1 / PLK-1
Human
IHC - Paraffin, Western blot, Immunoprecipitation, Flow Cytometry
Unconjugated
PLK1 / PLK-1 Rabbit Polyclonal Unconjugated Human IHC-P, WB C-Terminus No
Anti-PLK1 / PLK-1 Antibody (C-Terminus) LS-C99728
IHC Image
WB Image
Rabbit Polyclonal to Human PLK1 / PLK-1
Human
IHC - Paraffin, Western blot
Unconjugated
PLK1 / PLK-1 Rabbit Polyclonal Unconjugated Human, Mouse, Rat IHC, ELISA pThr210 No
Anti-PLK1 / PLK-1 Antibody (phospho-Thr210) LS-C199481
Rabbit Polyclonal (IgG) to Human PLK1 / PLK-1
Human, Mouse, Rat
IHC, ELISA
Unconjugated
PLK1 / PLK-1 Mouse Monoclonal 1D1 Unconjugated Human IHC, ICC, IF, WB, ELISA No
Anti-PLK1 / PLK-1 Antibody [clone 1D1] LS-C169107
IF Image
WB Image
Mouse Monoclonal [clone 1D1] (IgG1) to Human PLK1 / PLK-1
Human
IHC, ICC, Immunofluorescence, Western blot, ELISA
Unconjugated
PLK1 / PLK-1 Rabbit Polyclonal Unconjugated Human, Mouse, Rat IHC, IF, WB Asp204 No
Anti-PLK1 / PLK-1 Antibody (Asp204) LS-C176552
WB Image
Rabbit Polyclonal to Human PLK1 / PLK-1
Human, Mouse, Rat
IHC, Immunofluorescence, Western blot
Unconjugated
PLK1 / PLK-1 Rabbit Polyclonal FITC Conjugated Human IHC, WB, ELISA aa574-603 No
Anti-PLK1 / PLK-1 Antibody (aa574-603) (FITC) LS-C266559
Rabbit Polyclonal (IgG) to Human PLK1 / PLK-1
Human
IHC, Western blot, ELISA
FITC Conjugated
PLK1 / PLK-1 Rabbit Polyclonal HRP Conjugated Human IHC, WB, ELISA aa574-603 No
Anti-PLK1 / PLK-1 Antibody (aa574-603) (HRP) LS-C266560
Rabbit Polyclonal (IgG) to Human PLK1 / PLK-1
Human
IHC, Western blot, ELISA
HRP Conjugated
PLK1 / PLK-1 Rabbit Polyclonal PE Conjugated Human IHC, WB, ELISA aa574-603 No
Anti-PLK1 / PLK-1 Antibody (aa574-603) (PE) LS-C266566
Rabbit Polyclonal (IgG) to Human PLK1 / PLK-1
Human
IHC, Western blot, ELISA
PE Conjugated
PLK1 / PLK-1 Rabbit Polyclonal AP Conjugated Human IHC, WB, ELISA aa574-603 No
Anti-PLK1 / PLK-1 Antibody (aa574-603) (AP) LS-C266556
Rabbit Polyclonal (IgG) to Human PLK1 / PLK-1
Human
IHC, Western blot, ELISA
AP Conjugated
PLK1 / PLK-1 Rabbit Polyclonal APC Conjugated Human IHC, WB, ELISA aa574-603 No
Anti-PLK1 / PLK-1 Antibody (aa574-603) (APC) LS-C266557
Rabbit Polyclonal (IgG) to Human PLK1 / PLK-1
Human
IHC, Western blot, ELISA
APC Conjugated
PLK1 / PLK-1 Rabbit Polyclonal Biotin Conjugated Human IHC, WB, ELISA aa574-603 No
Anti-PLK1 / PLK-1 Antibody (aa574-603) (Biotin) LS-C266558
Rabbit Polyclonal (IgG) to Human PLK1 / PLK-1
Human
IHC, Western blot, ELISA
Biotin Conjugated
PLK1 / PLK-1 Mouse Monoclonal 8C12 Unconjugated Human ELISA No
Anti-PLK1 / PLK-1 Antibody [clone 8C12] LS-C174903
ELISA Image
ELISA Image
Mouse Monoclonal [clone 8C12] (IgG1) to Human PLK1 / PLK-1
Human
ELISA
Unconjugated
PLK1 / PLK-1 Mouse Monoclonal 2G12 Unconjugated Human IF, WB, IP, ELISA No
Anti-PLK1 / PLK-1 Antibody [clone 2G12] LS-C197924
IF Image
WB Image
Mouse Monoclonal [clone 2G12] (IgG1,k) to Human PLK1 / PLK-1
Human
Immunofluorescence, Western blot, Immunoprecipitation, ELISA
Unconjugated
PLK1 / PLK-1 Mouse Monoclonal 8C12 Unconjugated Human, Monkey, Dog IF, WB, IP, Flo No
Anti-PLK1 / PLK-1 Antibody [clone 8C12] LS-C114499
IF Image
Flo Image
Mouse Monoclonal [clone 8C12] (IgG1) to Human PLK1 / PLK-1
Human, Monkey, Dog
Immunofluorescence, Western blot, Immunoprecipitation, Flow Cytometry
Unconjugated
PLK1 / PLK-1 Rabbit Polyclonal Unconjugated Human, Mouse, Rat IF, WB, ELISA aa176-225 No
Anti-PLK1 / PLK-1 Antibody (aa176-225) LS-C117697
WB Image
Rabbit Polyclonal (IgG) to Human PLK1 / PLK-1
Human, Mouse, Rat
Immunofluorescence, Western blot, ELISA
Unconjugated
PLK1 / PLK-1 Mouse Monoclonal 6C6 Biotin Conjugated Human ELISA No
Anti-PLK1 / PLK-1 Antibody [clone 6C6] (Biotin) LS-C175038
ELISA Image
ELISA Image
Mouse Monoclonal [clone 6C6] (IgG1) to Human PLK1 / PLK-1
Human
ELISA
Biotin Conjugated
PLK1 / PLK-1 Rabbit Polyclonal Unconjugated Human WB pThr210 No
Anti-PLK1 / PLK-1 Antibody (phospho-Thr210) LS-C41309
ICC Image
WB Image
Rabbit Polyclonal (IgG) to Human PLK1 / PLK-1
Human
Western blot
Unconjugated
PLK1 / PLK-1 Mouse Monoclonal 1D4 Biotin Conjugated Human ELISA No
Anti-PLK1 / PLK-1 Antibody [clone 1D4] (Biotin) LS-C175036
ELISA Image
ELISA Image
Mouse Monoclonal [clone 1D4] (IgG1) to Human PLK1 / PLK-1
Human
ELISA
Biotin Conjugated
PLK1 / PLK-1 Mouse Monoclonal 5C1 Biotin Conjugated Human ELISA No
Anti-PLK1 / PLK-1 Antibody [clone 5C1] (Biotin) LS-C175037
ELISA Image
ELISA Image
Mouse Monoclonal [clone 5C1] (IgG1) to Human PLK1 / PLK-1
Human
ELISA
Biotin Conjugated
PLK1 / PLK-1 Mouse Monoclonal Unconjugated Human, Mouse, Rat WB, IP, ELISA No
Anti-PLK1 / PLK-1 Antibody LS-C62048
WB Image
Mouse Monoclonal (IgG1,k) to Human PLK1 / PLK-1
Human, Mouse, Rat
Western blot, Immunoprecipitation, ELISA
Unconjugated
PLK1 / PLK-1 Goat Polyclonal Unconjugated Human WB, ELISA aa287-298 No
Anti-PLK1 / PLK-1 Antibody (aa287-298) LS-C186577
WB Image
Goat Polyclonal to Human PLK1 / PLK-1
Human
Western blot, ELISA
Unconjugated
PLK1 / PLK-1 Mouse Monoclonal 3F8 Unconjugated Human, Mouse WB, IP No
Anti-PLK1 / PLK-1 Antibody [clone 3F8] LS-C40938
WB Image
Mouse Monoclonal [clone 3F8] (IgG1) to Human PLK1 / PLK-1
Human, Mouse
Western blot, Immunoprecipitation
Unconjugated
PLK1 / PLK-1 Rabbit Monoclonal EPR2612(2) Unconjugated Human WB pThr210 No
Anti-PLK1 / PLK-1 Antibody (phospho-Thr210) [clone EPR2612(2)] LS-C138420
WB Image
Rabbit Monoclonal [clone EPR2612(2)] to Human PLK1 / PLK-1
Human
Western blot
Unconjugated
PLK1 / PLK-1 Rabbit Polyclonal Unconjugated Human, Dog, Horse WB N-Terminus No
Anti-PLK1 / PLK-1 Antibody (N-Terminus) LS-C146512
WB Image
Rabbit Polyclonal to Human PLK1 / PLK-1
Human, Dog, Horse
Western blot
Unconjugated
PLK1 / PLK-1 Mouse Monoclonal 9E9 Unconjugated Human WB No
Anti-PLK1 / PLK-1 Antibody [clone 9E9] LS-C172325
WB Image
Mouse Monoclonal [clone 9E9] (IgG2b) to Human PLK1 / PLK-1
Human
Western blot
Unconjugated
PLK1 / PLK-1 Mouse Monoclonal 3F8 Unconjugated Human, Mouse, Rat WB Internal No
Anti-PLK1 / PLK-1 Antibody (Internal) [clone 3F8] LS-C63154
WB Image
Mouse Monoclonal [clone 3F8] (IgG1) to Human PLK1 / PLK-1
Human, Mouse, Rat
Western blot
Unconjugated
PLK1 / PLK-1 Rabbit Polyclonal Unconjugated Human, Mouse, Rat, Bovine, Dog, Xenopus WB pThr210 No
Anti-PLK1 / PLK-1 Antibody (phospho-Thr210) LS-C53476
WB Image
Rabbit Polyclonal
Human, Mouse, Rat, Bovine, Dog, Xenopus
Western blot
Unconjugated
PLK1 / PLK-1 Rabbit Polyclonal Unconjugated Human WB Ser482,486,490 No
Anti-PLK1 / PLK-1 Antibody (Ser482,486,490) LS-C195217
Rabbit Polyclonal to Human PLK1 / PLK-1
Human
Western blot
Unconjugated
PLK1 / PLK-1 Rabbit Polyclonal Unconjugated Mouse, Human, Rat, Drosophila WB pThr210 No
Anti-PLK1 / PLK-1 Antibody (phospho-Thr210) LS-C63162
Rabbit Polyclonal to Mouse PLK1 / PLK-1
Mouse, Human, Rat, Drosophila
Western blot
Unconjugated
PLK1 / PLK-1 Rabbit Polyclonal Unconjugated Human, Gorilla, Gibbon, Rabbit ELISA aa344-360 No
Anti-PLK1 / PLK-1 Antibody (aa344-360) LS-C148900
Rabbit Polyclonal to Human PLK1 / PLK-1
Human, Gorilla, Gibbon, Rabbit
ELISA
Unconjugated
PLK1 / PLK-1 Mouse Monoclonal Unconjugated Human, Mouse, Rat, Xenopus IF, WB, IP, ELISA aa402-603 No
Anti-PLK1 / PLK-1 Antibody (aa402-603) LS-C17320
Mouse Monoclonal (IgG) to Human PLK1 / PLK-1
Human, Mouse, Rat, Xenopus
Immunofluorescence, Western blot, Immunoprecipitation, ELISA
Unconjugated
PLK1 / PLK-1 Mouse Monoclonal Unconjugated Human IF, WB, IP, ELISA No
Anti-PLK1 / PLK-1 Antibody LS-C9649
Mouse Monoclonal (IgG1,k) to Human PLK1 / PLK-1
Human
Immunofluorescence, Western blot, Immunoprecipitation, ELISA
Unconjugated
PLK1 / PLK-1 Mouse Monoclonal Unconjugated Human IF, WB, IP, ELISA No
Anti-PLK1 / PLK-1 Antibody LS-C9648
Mouse Monoclonal (IgG1,k) to Human PLK1 / PLK-1
Human
Immunofluorescence, Western blot, Immunoprecipitation, ELISA
Unconjugated
PLK1 / PLK-1 Mouse Monoclonal HRP Conjugated Human ICC, WB, ELISA aa1-604 No
Anti-PLK1 / PLK-1 Antibody (aa1-604) (HRP) LS-C281096
Mouse Monoclonal (IgG1) to Human PLK1 / PLK-1
Human
ICC, Western blot, ELISA
HRP Conjugated
PLK1 / PLK-1 Mouse Monoclonal PE Conjugated Human ICC, WB, ELISA aa1-604 No
Anti-PLK1 / PLK-1 Antibody (aa1-604) (PE) LS-C281098
Mouse Monoclonal (IgG1) to Human PLK1 / PLK-1
Human
ICC, Western blot, ELISA
PE Conjugated
PLK1 / PLK-1 Mouse Monoclonal 1D4B Unconjugated Human, Mouse IF, WB, IP aa300-603 No
Anti-PLK1 / PLK-1 Antibody (aa300-603) [clone 1D4B] LS-C189750
Mouse Monoclonal [clone 1D4B] (IgG1,k) to Human PLK1 / PLK-1
Human, Mouse
Immunofluorescence, Western blot, Immunoprecipitation
Unconjugated
PLK1 / PLK-1 Rabbit Polyclonal Unconjugated Human IF, WB, IP aa325-375 No
Anti-PLK1 / PLK-1 Antibody (aa325-375) LS-C91988
Rabbit Polyclonal to Human PLK1 / PLK-1
Human
Immunofluorescence, Western blot, Immunoprecipitation
Unconjugated
PLK1 / PLK-1 Mouse Monoclonal AP Conjugated Human ICC, WB, ELISA aa1-604 No
Anti-PLK1 / PLK-1 Antibody (aa1-604) (AP) LS-C281094
Mouse Monoclonal (IgG1) to Human PLK1 / PLK-1
Human
ICC, Western blot, ELISA
AP Conjugated
PLK1 / PLK-1 Mouse Monoclonal APC Conjugated Human ICC, WB, ELISA aa1-604 No
Anti-PLK1 / PLK-1 Antibody (aa1-604) (APC) LS-C281095
Mouse Monoclonal (IgG1) to Human PLK1 / PLK-1
Human
ICC, Western blot, ELISA
APC Conjugated
PLK1 / PLK-1 Rabbit Polyclonal Unconjugated Human WB, IP aa1-50 No
Anti-PLK1 / PLK-1 Antibody (aa1-50) LS-C91987
Rabbit Polyclonal to Human PLK1 / PLK-1
Human
Western blot, Immunoprecipitation
Unconjugated
PLK1 / PLK-1 Mouse Monoclonal 13A215 FITC Conjugated Human Flo No
Anti-PLK1 / PLK-1 Antibody [clone 13A215] (FITC) LS-C183406
Mouse Monoclonal [clone 13A215] (IgG2b) to Human PLK1 / PLK-1
Human
Flow Cytometry
FITC Conjugated
PLK1 / PLK-1 Rabbit Polyclonal Unconjugated Xenopus, Axlotl WB pSer482/Ser486/Ser490 No
Anti-PLK1 / PLK-1 Antibody (phospho-Ser482/Ser486/Ser490) LS-C182543
Rabbit Polyclonal (IgG) to Xenopus PLK1 / PLK-1
Xenopus, Axlotl
Western blot
Unconjugated
PLK1 / PLK-1 Mouse Monoclonal 2A3 Unconjugated Human IHC-P, WB pThr210 Yes
Anti-PLK1 / PLK-1 Antibody (phospho-Thr210) [clone 2A3] IHC-plus™ LS-B1675
PublicationsPublications (1)
IHC Image
WB Image
Mouse Monoclonal [clone 2A3] (IgG1) to Human PLK1 / PLK-1
Human
IHC - Paraffin, Western blot
Unconjugated

Immunohistochemistry

Immunohistochemistry
Anti-PLK1 antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1675 concentration 15 ug/ml.

Western Blot

Western Blot
Panel A. Extracts from untreated Hela cells (Lane 1) or overnight nocodazole-treated Hela cells (Lane 2) were immunoprecipitated with the pan-PLK mAb (clone 3F8), resolved by electrophoresis, transferred to nitrocellulose and probed with mAb 2A3 reactive against Thr210-phosphorylated PLK-1. Proteins were visualized using an HRP goat anti-mouse secondary Ab and a chemiluminescence detection system. Panel B. Extracts from untreated Hela cells (Lane 1) or overnight nocodazole-treated Hela cells (Lane 2) were resolved by electrophoresis, transferred to nitrocellulose and probed with mAb 2A3 reactive against Thr210-phosphorylated PLK-1. Proteins were visualized using an HRP goat anti-mouse secondary and a chemiluminescence detection system.

Western Blot

Western Blot
 
 

Requested From: United States
Date Requested: 4/20/2014
Copyright © 2014 LifeSpan BioSciences, Inc. All Rights Reserved