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Anti-PDCD4 Antibody (phospho-Ser457) IHC-plus™ LS-B487

Ordering

Wt. Vol. Conc. Price
50 µg - 1 mg/ml $395

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

PDCD4 Antibody LS-B487 Images

[click image for a larger image and more details]
Immunohistochemistry
Anti-PDCD4 antibody IHC of human breast.
Western Blot
Anti-Pdcd4 pS457 - Western Blot.

100% Guaranteed 100% Guaranteed Publications Publications (1)
Rabbit Polyclonal to Human PDCD4
Human, Mouse, Rat, Xenopus
IHC - Paraffin, Western blot, ELISA
Unconjugated

Details

Human PDCD4
Rabbit
Human, Mouse, Rat, Xenopus (tested or 100% immunogen sequence identity)
Polyclonal
Immunoaffinity purified

Applications

IHC - Paraffin (1.25 - 2.5 µg/ml), Western blot (1:200 - 1:5000), ELISA (1:1500 - 1:7000)

Specificity and Use

PDCD4 antibody was raised against synthetic peptide from human PDCD4.
pSer457
Amino acids 447-465 of human Pdcd4 protein.
Immunohistochemistry: LS-B487 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B487 was determined to be 1.25-2.5 ug/ml.

Packaging

0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

Publications (1)

1
Up-regulation of miR-21 by HER2/neu signaling promotes cell invasion. Huang TH, Wu F, Loeb GB, Hsu R, Heidersbach A, Brincat A, Horiuchi D, Lebbink RJ, Mo YY, Goga A, McManus MT. The Journal of biological chemistry. 2009 284:18515-24. [PubMed:19419954] [PMC:PMC2709372]

About PDCD4

Q53EL6 NM_014456 NP_055271.2

PDCD4 Antibody, H731 Antibody, Nuclear antigen H731-like Antibody, Nuclear antigen H731 Antibody, Protein 197/15a Antibody

This gene encodes a protein localized to the nucleus in proliferating cells. Expression of this gene is modulated by cytokines in natural killer and T cells. The gene product is thought to play a role in apoptosis but the specific role has not yet been determined. Two transcripts encoding different isoforms have been identified. PDCD4 inhibits the translation initiation factor E1F4A.


PDCD4 Antibodies (31)

Target Reactivity Applications Host Clonality Clone Format Epitope Publications
PDCD4 Mouse Monoclonal K4C1 Unconjugated Human IHC-P, WB, ELISA aa1-469 No
Anti-PDCD4 Antibody (aa1-469) [clone K4C1] IHC-plus™ LS-B2949
IHC Image
IHC Image
Mouse Monoclonal [clone K4C1] (IgG1,k) to Human PDCD4
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated
PDCD4 Goat Polyclonal Unconjugated Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Mouse, Rat, Bat, Bovine, Dog, Goat, Guinea pig, Hamster, Horse, Rabbit, Chicken, Xenopus IHC-P, WB, ELISA aa210-221 No
Anti-PDCD4 Antibody (aa210-221) IHC-plus™ LS-B9458
IHC Image
WB Image
Goat Polyclonal to Human PDCD4
Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Mouse, Rat, Bat, Bovine, Dog, Goat, Guinea pig, Hamster, Horse, Rabbit, Chicken, Xenopus
IHC - Paraffin, Western blot, ELISA
Unconjugated
PDCD4 Rabbit Polyclonal Unconjugated Human, Mouse, Rat, Xenopus IHC-P, WB, ELISA C-Terminus Yes
Anti-PDCD4 Antibody (C-Terminus) IHC-plus™ LS-B65
PublicationsPublications (1)
IHC Image
IHC Image
Rabbit Polyclonal to Human PDCD4
Human, Mouse, Rat, Xenopus
IHC - Paraffin, Western blot, ELISA
Unconjugated
PDCD4 Rabbit Polyclonal Unconjugated Human, Gorilla, Orangutan, Monkey, Mouse, Rat, Bat, Bovine, Dog, Goat, Hamster, Horse, Pig, Rabbit, Chicken IHC-P, WB No
Anti-PDCD4 Antibody IHC-plus™ LS-B6949
IHC Image
WB Image
Rabbit Polyclonal (IgG) to Human PDCD4
Human, Gorilla, Orangutan, Monkey, Mouse, Rat, Bat, Bovine, Dog, Goat, Hamster, Horse, Pig, Rabbit, Chicken
IHC - Paraffin, Western blot
Unconjugated
PDCD4 Rabbit Polyclonal Unconjugated Human, Mouse, Rat IHC-P, WB C-Terminus No
Anti-PDCD4 Antibody (C-Terminus) IHC-plus™ LS-B1388
IHC Image
WB Image
Rabbit Polyclonal to Human PDCD4
Human, Mouse, Rat
IHC - Paraffin, Western blot
Unconjugated
PDCD4 Rabbit Polyclonal Unconjugated Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Dog IHC-P, ICC, WB, IP No
Anti-PDCD4 Antibody LS-C179904
IHC Image
IHC Image
Rabbit Polyclonal (IgG) to Human PDCD4
Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Dog
IHC - Paraffin, ICC, Western blot, Immunoprecipitation
Unconjugated
PDCD4 Rabbit Polyclonal Unconjugated Human, Mouse IHC-P, WB No
Anti-PDCD4 Antibody LS-C179905
IHC Image
IHC Image
Rabbit Polyclonal (IgG) to Human PDCD4
Human, Mouse
IHC - Paraffin, Western blot
Unconjugated
PDCD4 Mouse Monoclonal 9G6 Unconjugated Human, Mouse, Rat, Xenopus IHC, WB, ELISA pSer457 Yes
Anti-PDCD4 Antibody (phospho-Ser457) [clone 9G6] LS-C154004
PublicationsPublications (1)
IHC Image
WB Image
Mouse Monoclonal [clone 9G6] (IgG) to Human PDCD4
Human, Mouse, Rat, Xenopus
IHC, Western blot, ELISA
Unconjugated
PDCD4 Rabbit Monoclonal EPR3431 Unconjugated Human IHC-P, ICC, WB, IP, Flo N-Terminus No
Anti-PDCD4 Antibody (N-Terminus) [clone EPR3431] LS-C105569
IHC Image
WB Image
Rabbit Monoclonal [clone EPR3431] (IgG) to Human PDCD4
Human
IHC - Paraffin, ICC, Western blot, Immunoprecipitation, Flow Cytometry
Unconjugated
PDCD4 Rabbit Monoclonal EPR3432 Unconjugated Human, Mouse IHC-P, ICC, WB, Flo C-Terminus No
Anti-PDCD4 Antibody (C-Terminus) [clone EPR3432] LS-C105556
IHC Image
WB Image
Rabbit Monoclonal [clone EPR3432] (IgG) to Human PDCD4
Human, Mouse
IHC - Paraffin, ICC, Western blot, Flow Cytometry
Unconjugated
PDCD4 Rabbit Polyclonal Unconjugated Human, Mouse, Rat IHC, IF, WB Lys453 No
Anti-PDCD4 Antibody (Lys453) LS-C176644
IHC Image
WB Image
Rabbit Polyclonal to Human PDCD4
Human, Mouse, Rat
IHC, Immunofluorescence, Western blot
Unconjugated
PDCD4 Rabbit Polyclonal Unconjugated Human, Mouse, Rat IHC, WB, ELISA pSer457 No
Anti-PDCD4 Antibody (phospho-Ser457) LS-C199415
WB Image
Rabbit Polyclonal (IgG) to Human PDCD4
Human, Mouse, Rat
IHC, Western blot, ELISA
Unconjugated
PDCD4 Rabbit Polyclonal Unconjugated Human IHC-P, WB aa120-149 No
Anti-PDCD4 Antibody (aa120-149) LS-C163460
IHC Image
WB Image
Rabbit Polyclonal to Human PDCD4
Human
IHC - Paraffin, Western blot
Unconjugated
PDCD4 Rabbit Polyclonal Biotin Conjugated Human IHC, WB, ELISA aa120-149 No
Anti-PDCD4 Antibody (aa120-149) (Biotin) LS-C216650
Rabbit Polyclonal (IgG) to Human PDCD4
Human
IHC, Western blot, ELISA
Biotin Conjugated
PDCD4 Rabbit Polyclonal HRP Conjugated Human IHC, WB, ELISA aa120-149 No
Anti-PDCD4 Antibody (aa120-149) (HRP) LS-C216651
Rabbit Polyclonal (IgG) to Human PDCD4
Human
IHC, Western blot, ELISA
HRP Conjugated
PDCD4 Rabbit Polyclonal PE Conjugated Human IHC, WB, ELISA aa120-149 No
Anti-PDCD4 Antibody (aa120-149) (PE) LS-C216654
Rabbit Polyclonal (IgG) to Human PDCD4
Human
IHC, Western blot, ELISA
PE Conjugated
PDCD4 Rabbit Polyclonal FITC Conjugated Human IHC, WB, ELISA aa120-149 No
Anti-PDCD4 Antibody (aa120-149) (FITC) LS-C216649
Rabbit Polyclonal (IgG) to Human PDCD4
Human
IHC, Western blot, ELISA
FITC Conjugated
PDCD4 Rabbit Polyclonal Unconjugated Human IHC-P, WB, ELISA C-Terminus No
Anti-PDCD4 Antibody (C-Terminus) LS-C122296
Rabbit Polyclonal (IgG) to Human PDCD4
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated
PDCD4 Rabbit Polyclonal AP Conjugated Human IHC, WB, ELISA aa120-149 No
Anti-PDCD4 Antibody (aa120-149) (AP) LS-C216647
Rabbit Polyclonal (IgG) to Human PDCD4
Human
IHC, Western blot, ELISA
AP Conjugated
PDCD4 Rabbit Polyclonal APC Conjugated Human IHC, WB, ELISA aa120-149 No
Anti-PDCD4 Antibody (aa120-149) (APC) LS-C216648
Rabbit Polyclonal (IgG) to Human PDCD4
Human
IHC, Western blot, ELISA
APC Conjugated
PDCD4 Rabbit Polyclonal Unconjugated Human, Mouse, Rat IF, WB, ELISA aa420-469 No
Anti-PDCD4 Antibody (aa420-469) LS-C118014
WB Image
Rabbit Polyclonal (IgG) to Human PDCD4
Human, Mouse, Rat
Immunofluorescence, Western blot, ELISA
Unconjugated
PDCD4 Mouse Monoclonal 9G6 Biotin Conjugated Human, Mouse, Rat, Xenopus WB, IP, ELISA pSer457 Yes
Anti-PDCD4 Antibody (phospho-Ser457) [clone 9G6] (Biotin) LS-C153791
PublicationsPublications (1)
WB Image
Mouse Monoclonal [clone 9G6] (IgG) to Human PDCD4
Human, Mouse, Rat, Xenopus
Western blot, Immunoprecipitation, ELISA
Biotin Conjugated
PDCD4 Rabbit Polyclonal Unconjugated Human, Mouse, Rat WB, ELISA pSer67 No
Anti-PDCD4 Antibody (phospho-Ser67) LS-C199416
WB Image
Rabbit Polyclonal (IgG) to Human PDCD4
Human, Mouse, Rat
Western blot, ELISA
Unconjugated
PDCD4 Goat Polyclonal Unconjugated Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Mouse, Rat, Bovine, Dog, Goat, Guinea pig, Hamster, Horse, Rabbit WB, ELISA Internal No
Anti-PDCD4 Antibody (Internal) LS-C155028
WB Image
Goat Polyclonal to Human PDCD4
Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Mouse, Rat, Bovine, Dog, Goat, Guinea pig, Hamster, Horse, Rabbit
Western blot, ELISA
Unconjugated
PDCD4 Rabbit Polyclonal Unconjugated Human, Mouse, Bovine, Dog, Goat, Guinea pig, Pig, Rabbit WB N-Terminus No
Anti-PDCD4 Antibody (N-Terminus) LS-C31656
WB Image
Rabbit Polyclonal (IgG) to Human PDCD4
Human, Mouse, Bovine, Dog, Goat, Guinea pig, Pig, Rabbit
Western blot
Unconjugated
PDCD4 Rabbit Polyclonal Unconjugated Human, Mouse, Bovine, Dog, Goat, Guinea pig, Pig, Rabbit WB N-Terminus No
Anti-PDCD4 Antibody (N-Terminus) LS-C29959
WB Image
Rabbit Polyclonal (IgG) to Human PDCD4
Human, Mouse, Bovine, Dog, Goat, Guinea pig, Pig, Rabbit
Western blot
Unconjugated
PDCD4 Mouse Monoclonal Pk4C1AT Unconjugated Human WB, ELISA aa1-469 No
Anti-PDCD4 Antibody (aa1-469) [clone Pk4C1AT] LS-C204717
Mouse Monoclonal [clone Pk4C1AT] (IgG1,k) to Human PDCD4
Human
Western blot, ELISA
Unconjugated
PDCD4 Mouse Monoclonal Unconjugated Human WB, ELISA aa1-469 No
Anti-PDCD4 Antibody (aa1-469) LS-C74212
Mouse Monoclonal (IgG1,k) to Human PDCD4
Human
Western blot, ELISA
Unconjugated
PDCD4 Rabbit Polyclonal Unconjugated Human WB, IP aa419-469 No
Anti-PDCD4 Antibody (aa419-469) LS-C93128
Rabbit Polyclonal to Human PDCD4
Human
Western blot, Immunoprecipitation
Unconjugated
PDCD4 Rabbit Polyclonal Unconjugated Human IP aa100-150 No
Anti-PDCD4 Antibody (aa100-150) LS-C93127
Rabbit Polyclonal to Human PDCD4
Human
Immunoprecipitation
Unconjugated
PDCD4 Rabbit Polyclonal Unconjugated Human, Mouse, Rat, Xenopus IHC-P, WB, ELISA pSer457 Yes
Anti-PDCD4 Antibody (phospho-Ser457) IHC-plus™ LS-B487
PublicationsPublications (1)
IHC Image
WB Image
Rabbit Polyclonal to Human PDCD4
Human, Mouse, Rat, Xenopus
IHC - Paraffin, Western blot, ELISA
Unconjugated

Immunohistochemistry

Immunohistochemistry
Anti-PDCD4 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B487 concentration 5 ug/ml.

Western Blot

Western Blot
Anti-Pdcd4 pS457 - Western Blot. Western blot of affinity purified anti-Pdcd4 pS457 antibody shows detection of Pdcd4 phosphorylated at Ser 457 (arrowheads). Lanes 1 & 2 each contain 100 ng recombinant Pdcd4. Lanes 3 & 4 each contain 30 ug of whole cell extract from 293 HEK cells treated with 20 nM TPA and MG132 proteosome inhibitor for 8 hours. The signal can be competed off with peptide phosphorylated at Ser 457 (Lanes 2 & 4). Personal Communication, M Young & A Jansen, NCI, Bethesda, MD.

Requested From: United States
Date Requested: 4/18/2014
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