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Anti-MYL2 Antibody [clone AT3B2] IHC-plus™ LS-B3584

Ordering

Wt. Vol. Conc. Price
- 50 µl 1 mg/ml $395

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

MYL2 Antibody LS-B3584 Images

[click image for a larger image and more details]
Immunohistochemistry
Anti-MYL2 antibody IHC of human skeletal muscle.
Western Blot
Western analysis of mouse liver extracts

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Mouse Monoclonal [clone AT3B2] (IgG2b,k) to Human MYL2
Human, Mouse, Rat
IHC - Paraffin, Western blot, ELISA
Unconjugated

Details

Human MYL2
Mouse
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
IgG2b,k Monoclonal [AT3B2]
Protein G purified

Applications

IHC - Paraffin (5 µg/ml), Western blot (1:1000 - 1:2000), ELISA

Specificity and Use

MYL2 antibody was raised against recombinant human MYL2 (1-166aa) purified from E. coli
human MYL2 (1-166aa)
Immunohistochemistry: LS-B3584 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3584 was determined to be 5 ug/ml. Western Blot: Extracts of Rat heart (60ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human MYL2 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Packaging

PBS, pH 7.4, 0.1% sodium azide
Aliquot and store at -20°C. Minimize freezing and thawing.
For research use only.

About MYL2

P10916 NM_000432 NP_000423.2

MYL2 Antibody, MLC2 Antibody, Myosin light chain 2 Antibody, MLC-2 Antibody, RLC of myosin Antibody, CMH10 Antibody, MLC-2v Antibody

MYL2 encodes the regulatory light chain associated with cardiac myosin beta (or slow) heavy chain. Ca+ triggers the phosphorylation of regulatory light chain that in turn triggers contraction. Mutations in MYL2 are associated with mid-left ventricular chamber type hypertrophic cardiomyopathy.


MYL2 Antibodies (10)

Target Reactivity Applications Host Clonality Clone Format Epitope Publications
MYL2 Rabbit Monoclonal EPR3741 Unconjugated Human, Mouse, Rat IHC-P, WB, IP Internal No
Anti-MYL2 Antibody (Internal) [clone EPR3741] IHC-plus™ LS-B8107
IHC Image
WB Image
Rabbit Monoclonal [clone EPR3741] (IgG) to Human MYL2
Human, Mouse, Rat
IHC - Paraffin, Western blot, Immunoprecipitation
Unconjugated
MYL2 Mouse Monoclonal 7C9 Unconjugated Human IHC-P, WB, ELISA No
Anti-MYL2 Antibody [clone 7C9] IHC-plus™ LS-B8057
IHC Image
WB Image
Mouse Monoclonal [clone 7C9] (IgG1) to Human MYL2
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated
MYL2 Rabbit Monoclonal EPR3740 Unconjugated Human, Mouse, Rat IHC-P, WB Internal No
Anti-MYL2 Antibody (Internal) [clone EPR3740] LS-C137797
IHC Image
WB Image
Rabbit Monoclonal [clone EPR3740] to Human MYL2
Human, Mouse, Rat
IHC - Paraffin, Western blot
Unconjugated
MYL2 Mouse Monoclonal 3B9-B4 Unconjugated Human IF, WB, IP, ELISA No
Anti-MYL2 Antibody [clone 3B9-B4] LS-C197730
IF Image
IP Image
Mouse Monoclonal [clone 3B9-B4] (IgG1,k) to Human MYL2
Human
Immunofluorescence, Western blot, Immunoprecipitation, ELISA
Unconjugated
MYL2 Rabbit Polyclonal Unconjugated Rat WB No
Anti-MYL2 Antibody LS-C175826
WB Image
Rabbit Polyclonal to Rat MYL2
Rat
Western blot
Unconjugated
MYL2 Mouse Monoclonal PAT3B2AT Unconjugated Human WB, ELISA aa1-166 No
Anti-MYL2 Antibody (aa1-166) [clone PAT3B2AT] LS-C204738
Mouse Monoclonal [clone PAT3B2AT] (IgG2b,k) to Human MYL2
Human
Western blot, ELISA
Unconjugated
MYL2 Mouse Monoclonal Unconjugated Human WB, ELISA aa1-166 No
Anti-MYL2 Antibody (aa1-166) LS-C130937
Mouse Monoclonal (IgG2b,k) to Human MYL2
Human
Western blot, ELISA
Unconjugated
MYL2 Rabbit Polyclonal Unconjugated Human, Chimpanzee, Mouse, Rat, Dog, Horse, Xenopus WB No
Anti-MYL2 Antibody LS-C183976
Rabbit Polyclonal (IgG) to Human MYL2
Human, Chimpanzee, Mouse, Rat, Dog, Horse, Xenopus
Western blot
Unconjugated
MYL2 Rabbit Polyclonal Unconjugated Mouse, Human, Chimpanzee, Monkey, Rat, Bovine, Dog, Horse, Pig, Sheep, Chicken, Xenopus, Zebrafish WB No
Anti-MYL2 Antibody LS-C130939
Rabbit Polyclonal (IgG) to Mouse MYL2
Mouse, Human, Chimpanzee, Monkey, Rat, Bovine, Dog, Horse, Pig, Sheep, Chicken, Xenopus, Zebrafish
Western blot
Unconjugated
MYL2 Mouse Monoclonal AT3B2 Unconjugated Human, Mouse, Rat IHC-P, WB, ELISA No
Anti-MYL2 Antibody [clone AT3B2] IHC-plus™ LS-B3584
IHC Image
WB Image
Mouse Monoclonal [clone AT3B2] (IgG2b,k) to Human MYL2
Human, Mouse, Rat
IHC - Paraffin, Western blot, ELISA
Unconjugated

Immunohistochemistry

Immunohistochemistry
Anti-MYL2 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3584 concentration 5 ug/ml.

Western Blot

Western Blot
Western analysis of mouse liver extracts

Requested From: United States
Date Requested: 4/20/2014
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