Mouse Monoclonal [clone AT3B2] (IgG2b,k) to Human MYL2
Human, Mouse, Rat
IHC - Paraffin, Western blot, ELISA
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
IgG2b,k Monoclonal [AT3B2]
Protein G purified
IHC - Paraffin (5 µg/ml), Western blot (1:1000 - 1:2000), ELISA
Specificity and Use
MYL2 antibody was raised against recombinant human MYL2 (1-166aa) purified from E. coli
human MYL2 (1-166aa)
Immunohistochemistry: LS-B3584 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3584 was determined to be 5 ug/ml. Western Blot: Extracts of Rat heart (60ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human MYL2 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
PBS, pH 7.4, 0.1% sodium azide
Aliquot and store at -20°C. Minimize freezing and thawing.
MYL2 encodes the regulatory light chain associated with cardiac myosin beta (or slow) heavy chain. Ca+ triggers the phosphorylation of regulatory light chain that in turn triggers contraction. Mutations in MYL2 are associated with mid-left ventricular chamber type hypertrophic cardiomyopathy.