Rat Monoclonal [clone C9B7W] (IgG1) to Mouse LAG3 / LAG-3 / CD223
Mouse LAG3 / LAG-3 / CD223
Mouse (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [C9B7W]
R. Phycoerythrin (RPE)
Protein G purified
Flow Cytometry (1:1 - 1:5)
Specificity and Use
LAG3 / LAG-3 / CD223 antibody was raised against murine CD223 Ig fusion protein.
Recognizes murine lymphocyte activation gene-3 (LAG-3), a 70kD activation-induced cell surface molecule that is also referred to as CD223. Murine CD223 is expressed on activated CD4 positive and CD8 positive alpha/beta T lymphocytes and a subset of natural killer (NK) cells. CD223 binds to MHC class II molecules with high affinity and is reported to negatively regulate T cell homeostasis and T cell expansion. Recognizes an epitope within the D2 domain of CD223. The antibody is reported to block the in vitro function of murine LAG-3 but studies suggest that the antibody does not block binding of LAG-3 to MHC class II. XXX recommend the use of MCA2386EL for this purpose.
Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100 ul. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors.
Lymphocyte-Activation Protein 3 (LAG3) belongs to Ig superfamily and contains 4 extracellular Ig-like domains. The LAG3 gene contains 8 exons. The sequence data, exon/intron organization, and chromosomal localization all indicate a close relationship of LAG3 to CD4.