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Anti-LAG3 / LAG-3 / CD223 Antibody [clone C9B7W] (RPE) LS-C44775

Ordering

Wt. Vol. Conc. Price
- 100 tst - $245

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Rat Monoclonal [clone C9B7W] (IgG1) to Mouse LAG3 / LAG-3 / CD223
Mouse
Flow Cytometry
RPE Conjugated

Details

Mouse LAG3 / LAG-3 / CD223
Rat
Mouse (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [C9B7W]
R. Phycoerythrin (RPE)
Protein G purified

Applications

Flow Cytometry (1:1 - 1:5)

Specificity and Use

LAG3 / LAG-3 / CD223 antibody was raised against murine CD223 Ig fusion protein.
Recognizes murine lymphocyte activation gene-3 (LAG-3), a 70kD activation-induced cell surface molecule that is also referred to as CD223. Murine CD223 is expressed on activated CD4 positive and CD8 positive alpha/beta T lymphocytes and a subset of natural killer (NK) cells. CD223 binds to MHC class II molecules with high affinity and is reported to negatively regulate T cell homeostasis and T cell expansion. Recognizes an epitope within the D2 domain of CD223. The antibody is reported to block the in vitro function of murine LAG-3 but studies suggest that the antibody does not block binding of LAG-3 to MHC class II. XXX recommend the use of MCA2386EL for this purpose.
Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100 ul. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors.

Packaging

Lyophilized, PBS, pH 7.4, 0.09% sodium azide, 1% BSA.
100 tst Distilled Water.
For research use only.

About LAG3 / LAG-3 / CD223

P18627 NM_002286 P18627

LAG3 Antibody, CD223 antigen Antibody, LAG-3 Antibody, Protein FDC Antibody, CD223 Antibody, FDC Antibody, Lymphocyte-activation gene 3 Antibody

Lymphocyte-Activation Protein 3 (LAG3) belongs to Ig superfamily and contains 4 extracellular Ig-like domains. The LAG3 gene contains 8 exons. The sequence data, exon/intron organization, and chromosomal localization all indicate a close relationship of LAG3 to CD4.


LAG3 / LAG-3 / CD223 Antibodies (20)

Target Reactivity Applications Host Clonality Clone Format Epitope Publications
LAG3 / LAG-3 / CD223 Mouse Monoclonal 17B4 FITC Conjugated Human IHC-P, Flo N-Terminus No
Anti-LAG3 / LAG-3 / CD223 Antibody (N-Terminus) [clone 17B4] (FITC) IHC-plus™ LS-B2237
IHC Image
Mouse Monoclonal [clone 17B4] (IgG1) to Human LAG3 / LAG-3 / CD223
Human
IHC - Paraffin, Flow Cytometry
FITC Conjugated
LAG3 / LAG-3 / CD223 Mouse Monoclonal 17B4 Unconjugated Human ICC, WB No
Anti-LAG3 / LAG-3 / CD223 Antibody [clone 17B4] LS-C18692
Mouse Monoclonal [clone 17B4] (IgG1) to Human LAG3 / LAG-3 / CD223
Human
ICC, Western blot
Unconjugated
LAG3 / LAG-3 / CD223 Goat Polyclonal APC Conjugated Human Flo No
Anti-LAG3 / LAG-3 / CD223 Antibody (APC) LS-C183944
Goat Polyclonal (IgG) to Human LAG3 / LAG-3 / CD223
Human
Flow Cytometry
APC Conjugated
LAG3 / LAG-3 / CD223 Rat Monoclonal Unconjugated Mouse Flo No
Anti-LAG3 / LAG-3 / CD223 Antibody LS-C44774
Rat Monoclonal (IgG1) to Mouse LAG3 / LAG-3 / CD223
Mouse
Flow Cytometry
Unconjugated
LAG3 / LAG-3 / CD223 Mouse Monoclonal 17B4 Biotin Conjugated Human ELISA No
Anti-LAG3 / LAG-3 / CD223 Antibody [clone 17B4] (Biotin) LS-C18691
Mouse Monoclonal [clone 17B4] (IgG1) to Human LAG3 / LAG-3 / CD223
Human
ELISA
Biotin Conjugated
LAG3 / LAG-3 / CD223 Rabbit Polyclonal Unconjugated Human IHC-P, WB aa110-140 No
Anti-LAG3 / LAG-3 / CD223 Antibody (aa110-140) LS-C165666
IHC Image
WB Image
Rabbit Polyclonal to Human LAG3 / LAG-3 / CD223
Human
IHC - Paraffin, Western blot
Unconjugated
LAG3 / LAG-3 / CD223 Mouse Monoclonal 11E3 Unconjugated Human, Monkey IHC-Fr, ICC, WB, IP, ELISA No
Anti-LAG3 / LAG-3 / CD223 Antibody [clone 11E3] LS-C18690
Mouse Monoclonal [clone 11E3] (IgG1) to Human LAG3 / LAG-3 / CD223
Human, Monkey
IHC - Frozen, ICC, Western blot, Immunoprecipitation, ELISA
Unconjugated
LAG3 / LAG-3 / CD223 Rabbit Polyclonal Unconjugated Human WB C-Terminus No
Anti-LAG3 / LAG-3 / CD223 Antibody (C-Terminus) LS-C116989
WB Image
Rabbit Polyclonal to Human LAG3 / LAG-3 / CD223
Human
Western blot
Unconjugated
LAG3 / LAG-3 / CD223 Rat Monoclonal Unconjugated, Low Endotoxin Mouse Flo, Func No
Anti-LAG3 / LAG-3 / CD223 Antibody LS-C44773
Rat Monoclonal (IgG1) to Mouse LAG3 / LAG-3 / CD223
Mouse
Flow Cytometry, Functional Assay
Unconjugated, Low Endotoxin
LAG3 / LAG-3 / CD223 Rabbit Polyclonal FITC Conjugated Human WB, ELISA Internal No
Anti-LAG3 / LAG-3 / CD223 Antibody (Internal) (FITC) LS-C278830
Rabbit Polyclonal (IgG) to Human LAG3 / LAG-3 / CD223
Human
Western blot, ELISA
FITC Conjugated
LAG3 / LAG-3 / CD223 Rabbit Polyclonal HRP Conjugated Human WB, ELISA Internal No
Anti-LAG3 / LAG-3 / CD223 Antibody (Internal) (HRP) LS-C278831
Rabbit Polyclonal (IgG) to Human LAG3 / LAG-3 / CD223
Human
Western blot, ELISA
HRP Conjugated
LAG3 / LAG-3 / CD223 Rabbit Polyclonal PE Conjugated Human WB, ELISA Internal No
Anti-LAG3 / LAG-3 / CD223 Antibody (Internal) (PE) LS-C278832
Rabbit Polyclonal (IgG) to Human LAG3 / LAG-3 / CD223
Human
Western blot, ELISA
PE Conjugated
LAG3 / LAG-3 / CD223 Rabbit Polyclonal Biotin Conjugated Human WB, ELISA Internal No
Anti-LAG3 / LAG-3 / CD223 Antibody (Internal) (Biotin) LS-C278829
Rabbit Polyclonal (IgG) to Human LAG3 / LAG-3 / CD223
Human
Western blot, ELISA
Biotin Conjugated
LAG3 / LAG-3 / CD223 Rat Monoclonal Unconjugated Mouse Flo, Func No
Anti-LAG3 / LAG-3 / CD223 Antibody LS-C37140
Rat Monoclonal (IgG1) to Mouse LAG3 / LAG-3 / CD223
Mouse
Flow Cytometry, Functional Assay
Unconjugated
LAG3 / LAG-3 / CD223 Rabbit Polyclonal AP Conjugated Human WB, ELISA Internal No
Anti-LAG3 / LAG-3 / CD223 Antibody (Internal) (AP) LS-C278824
Rabbit Polyclonal (IgG) to Human LAG3 / LAG-3 / CD223
Human
Western blot, ELISA
AP Conjugated
LAG3 / LAG-3 / CD223 Rabbit Polyclonal APC Conjugated Human WB, ELISA Internal No
Anti-LAG3 / LAG-3 / CD223 Antibody (Internal) (APC) LS-C278827
Rabbit Polyclonal (IgG) to Human LAG3 / LAG-3 / CD223
Human
Western blot, ELISA
APC Conjugated
LAG3 / LAG-3 / CD223 Goat Polyclonal PE Conjugated Mouse Flo Extracellular Domain No
Anti-LAG3 / LAG-3 / CD223 Antibody (Extracellular Domain) (PE) LS-C130399
Goat Polyclonal (IgG) to Mouse LAG3 / LAG-3 / CD223
Mouse
Flow Cytometry
PE Conjugated
LAG3 / LAG-3 / CD223 Goat Polyclonal PerCP-eFluor/PerCP Conjugated Human Flo Extracellular Domain No
Anti-LAG3 / LAG-3 / CD223 Antibody (Extracellular Domain) (PerCP-eFluor, PerCP) LS-C130398
Goat Polyclonal (IgG) to Human LAG3 / LAG-3 / CD223
Human
Flow Cytometry
PerCP-eFluor/PerCP Conjugated
LAG3 / LAG-3 / CD223 Rat Monoclonal 1D4B Unconjugated Mouse Flo aa23-442 No
Anti-LAG3 / LAG-3 / CD223 Antibody (aa23-442) [clone 1D4B] LS-C189811
Rat Monoclonal [clone 1D4B] (IgG2b) to Mouse LAG3 / LAG-3 / CD223
Mouse
Flow Cytometry
Unconjugated
LAG3 / LAG-3 / CD223 Rat Monoclonal C9B7W RPE Conjugated Mouse Flo No
Anti-LAG3 / LAG-3 / CD223 Antibody [clone C9B7W] (RPE) LS-C44775
Rat Monoclonal [clone C9B7W] (IgG1) to Mouse LAG3 / LAG-3 / CD223
Mouse
Flow Cytometry
RPE Conjugated

Requested From: United States
Date Requested: 4/23/2014
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