Target
Human IL6 / Interleukin 6
Synonyms
IL6 | B-cell stimulatory factor 2 | BSF-2 | BSF2 | CTL differentiation factor | Hybridoma growth factor | IFN-beta-2 | Interleukin-6 | Interleukin BSF-2 | IFNB2 | B-cell differentiation factor | CDF | HSF | IL-6
Reactivity
Human
(tested or 100% immunogen sequence identity)
Conjugations
Horseradish Peroxidase
Immunogen
This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant human IL-6 produced in E.coli.
Specificity
This antibody will recognize both native and recombinant human Il-6 in cell supernatants and certain body fluids. In Western blot analysis of natural cell products or human body fluids, multiple bands of IL-6 will appear due to the variable amount of glycosylation on the molecule. A control of similarly diluted normal rabbit IgG is recommended. This product has minimal reactivity with mouse IL-6.
Applications
- IHC (1:500 - 1:2500)
- Western blot (1:1000 - 1:5000)
- ELISA (1:10000 - 1:50000)
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Usage
This purified antibody has been tested for use in ELISA and western blotting. Reactivity is also expected in neutralizations, radioimmunoassay and immunohistochemistry. The endotoxin content is estimated to be <10 pg/ul by the LAL method. By western blot a band approximately 23.7 kD in size corresponding to native human IL-6 protein is expected in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Gentamicin. Do NOT add Sodium Azide
Reconstitution
Reconstitute in 100 µL of deionized water.
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About IL6 / Interleukin 6