Target
Human IL6 / Interleukin 6
Synonyms
IL6 | B-cell stimulatory factor 2 | BSF-2 | BSF2 | CTL differentiation factor | Hybridoma growth factor | IFN-beta-2 | Interleukin-6 | Interleukin BSF-2 | IFNB2 | B-cell differentiation factor | CDF | HSF | IL-6
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG2a,k
Monoclonal
Immunogen
COS-7-expressed recombinant human IL-6.
Specificity
Recognizes human Interleukin-6 (IL-6).
Applications
- Western blot
- Immunoprecipitation
- ELISA
Usage
ELISA Detection: Monoclonal antibody is useful as a detection antibody in a sandwich ELISA for quantifying human IL-6 protein levels.1-3 Biotinylated antibody should be paired with purified antibody as the capture antibody, with purified recombinant human IL-6 as the standard. Western Blot: The purified antibody is also useful for immunoprecipitation and Western blotting applications. For Western blotting, a concentration of 1-5 ug/ml (in conjunction with AP-labeled Goat Anti-Rat-Ig) has been found to enable detection of 100 ng/lane of recombinant human IL-6 under reducing conditions. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
PBS, Sodium Azide
Storage
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About IL6 / Interleukin 6