Human, Gorilla, Orangutan, Gibbon, Monkey, Mouse, Rat, Bat, Bovine, Dog, Horse, Pig
IHC - Paraffin, Western blot, ELISA
Human, Gorilla, Orangutan, Gibbon, Monkey, Mouse, Rat, Bat, Bovine, Dog, Horse, Pig (tested or 100% immunogen sequence identity)
Rabbit, Chicken (at least 90% immunogen sequence identity)
IHC - Paraffin (5 µg/ml), Western blot (1 - 3 µg/ml), ELISA (1:1000)
Specificity and Use
GPX3 antibody was raised against synthetic peptide C-NQFGKQEPGENSE from an internal region of human GPX3 (NP_002075.2). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bat, Bovine, Horse, Pig (100%); Rabbit, Opossum, Chicken (92%); Hamster, Zebrafish, Pseudomonas (85%).
Human GPX3. This antibody may cross-react with epididymis-specific GPX5 and olfactory epithelium-speciific GPX6
Immunohistochemistry: LS-B3036 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3036 was determined to be 5 ug/ml.
Glutathione peroxidase catalyzes the reduction of hydrogen peroxide, organic hydroperoxide, and lipid peroxides by reduced glutathione and functions in the protection of cells against oxidative damage. Human plasma glutathione peroxidase has been shown to be a selenium-containing enzyme. GPX3 expression appears to be tissue-specific.