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Anti-EPB42 Antibody (aa448-497) IHC-plus™ LS-B1526

Ordering

Wt. Vol. Conc. Price
50 µg 50 µl 1 mg/ml $395

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866-206-6909
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EPB42 Antibody LS-B1526 Images

[click image for a larger image and more details]
Immunohistochemistry
Anti-EPB42 / Pallidin antibody IHC of human placenta.
Western Blot
EPB42 / Pallidin antibody LS-B1526 Western blot of Fetal liver lysate.
Western Blot
Fetal Liver Lysate
 

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Rabbit Polyclonal (IgG) to Human EPB42
Human, Chimpanzee, Gibbon, Monkey, Rat, Bat, Guinea pig
IHC - Paraffin, Western blot
Unconjugated

Details

Human EPB42
Rabbit
Human, Chimpanzee, Gibbon, Monkey, Rat, Bat, Guinea pig (tested or 100% immunogen sequence identity)
Mouse, Dog, Horse, Rabbit (at least 90% immunogen sequence identity)
IgG Polyclonal
Immunoaffinity purified

Applications

IHC - Paraffin (5 µg/ml), Western blot (0.2 - 1 µg/ml)

Specificity and Use

EPB42 antibody was raised against synthetic peptide located between aa448-497 of human EPB42 (P16452-2, NP_000110). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset, Rat, Bat (100%); Mouse, Dog, Rabbit, Horse, Zebra finch (92%); Galago (84%); Chicken (80%).
LS-E11954 - Lyophilized - 100 µg - $145.00
aa448-497
Human EPB42 / Pallidin
Immunohistochemistry: LS-B1526 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1526 was determined to be 5 ug/ml.

Packaging

Lyophilized from PBS with 2% sucrose
50 µl After adding water, will consist of PBS buffer with 2% sucrose
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About EPB42

P16452 NM_000119 NP_000110.2

EPB42 Antibody, E42P Antibody, Erythrocyte protein 4.2 Antibody, p4.2 Antibody, Pallidin Antibody, SPH5 Antibody, Pa Antibody

Erythrocyte membrane protein band 4.2 is an ATP-binding protein which may regulate the association of protein 3 with ankyrin. It probably has a role in erythrocyte shape and mechanical property regulation. Mutations in the EPB42 gene are associated with recessive spherocytic elliptocytosis and recessively transmitted hereditary hemolytic anemia.

Immunohistochemistry

Immunohistochemistry
Anti-EPB42 / Pallidin antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1526 concentration 5 ug/ml.

Western Blot

Western Blot
EPB42 / Pallidin antibody LS-B1526 Western blot of Fetal liver lysate.

Western Blot

Western Blot
Fetal Liver Lysate
 

Requested From: United States
Date Requested: 10/25/2014

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