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Anti-DAXX Antibody (aa261-274) IHC-plus™ LS-B363

Ordering

Wt. Vol. Conc. Price
50 µg - 1 mg/ml $395

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
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DAXX Antibody LS-B363 Images

[click image for a larger image and more details]
Immunohistochemistry
Anti-DAXX antibody IHC of human kidney.
Western Blot
Anti-DAXX Antibody - Western Blot.

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Rabbit Polyclonal to Human DAXX
Human, Mouse, Rat
IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Unconjugated

Details

Human DAXX
Rabbit
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Polyclonal
Immunoaffinity purified

Applications

IHC - Paraffin (2.5 µg/ml), Immunofluorescence, Western blot (1:200 - 1:2000), ELISA (1:7000 - 1:45000)

Specificity and Use

DAXX antibody was raised against synthetic peptide from human Daxx.
aa261-274
Amino acids 261-274 of human DAXX protein.
Immunohistochemistry: LS-B363 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B363 was determined to be 2.5 ug/ml.

Packaging

0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
+4°C or -20°C, Avoid repeated freezing and thawing.
For research use only.

About DAXX

Q9UER7 NM_001350 NP_001341.1

DAXX Antibody, BING2 Antibody, CENP-C binding protein Antibody, DAP6 Antibody, Death-associated protein 6 Antibody, EAP1 Antibody, Fas-binding protein Antibody, HDaxx Antibody, ETS1-associated protein 1 Antibody

Apoptosis, or programmed cell death, occurs during normal cellular differentiation and development of multicellular organisms. Apoptosis is induced by certain cytokines including TNF and Fas ligand of the TNF family through their death domain containing receptors, TNFR1 and Fas. Cell death signals are transduced by death domain (DD)- containing adapter molecules and members of the ICE/CED-3 protease family.

Immunohistochemistry

Immunohistochemistry
Anti-DAXX antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B363 concentration 5 ug/ml.

Western Blot

Western Blot
Anti-DAXX Antibody - Western Blot. Western blot of affinity purified anti-DAXX antibody shows detection of DAXX in RWPE-1 cell extracts. The membrane was probed with the primary antibody diluted to 1:200, and further probed with peroxidase conjugated anti-Rabbit IgG at a 1:25000 dilution. Personal Communication, Jie LIU, CCR-NCI, Bethesda, MD.

Requested From: United States
Date Requested: 8/23/2014

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