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Anti-Complement C3 Antibody IHC-plus™ LS-B2545

Ordering

Wt. Vol. Conc. Price
- 50 µl 1 mg/ml $395

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Complement C3 Antibody LS-B2545 Images

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Immunohistochemistry
Anti-Complement C3 antibody IHC of human liver.

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Mouse Monoclonal (IgG1,k) to Human Complement C3
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated

Details

Human Complement C3
Mouse
Human (tested or 100% immunogen sequence identity)
IgG1,k Monoclonal
Protein A/G purified

Applications

IHC - Paraffin (10 µg/ml), Western blot, ELISA (1:30000)

Specificity and Use

Complement C3 antibody was raised against c3 isolated from human plasma.
Recognizes Complement component C3 (human). Epitope specificity differs from that of clones 6F66 and 6F63 but slightly overlaps as determined by inhibition ELISA. Reacts strongly with C3. Strong reaction is seen in ELISA with C3 directly coated onto the microtiter well, and also when tested in sandwich ELISA in combination with a polyclonal antibody against C3. In Western blotting after SDS-PAGE, this antibody reacts with C3 in reduced as well as non-reduced forms.
Immunohistochemistry: LS-B2545 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2545 was determined to be 10 ug/ml.

Packaging

PBS, pH 7.4, 15 mM sodium azide
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About Complement C3

P01024 NM_000064 NP_000055.2

C3 Antibody, AHUS5 Antibody, ARMD9 Antibody, Complement component C3 Antibody, CPAMD1 Antibody, Complement C3 Antibody, ASP Antibody, Complement component 3 Antibody

Complement component C3 plays a central role in the activation of complement system. Its activation is required for both classical and alternative complement activation pathways. People with C3 deficiency are susceptible to bacteria infection.

Immunohistochemistry

Immunohistochemistry
Anti-Complement C3 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2545 concentration 10 ug/ml.
 

Requested From: United States
Date Requested: 7/24/2014
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