Target
Human Complement C3
Synonyms
C3 | AHUS5 | ARMD9 | Complement component C3 | CPAMD1 | Complement C3 | ASP | Complement component 3
Reactivity
Human, Guinea pig, Rabbit
(tested or 100% immunogen sequence identity)
Clonality
IgG2b
Monoclonal
Conjugations
Unconjugated
Purification
Protein A purified
Immunogen
Human complement component C3.
Specificity
Recognizes a C3a-determinant (C3 alpha-chain) which is present also on native C3. Allows discrimination of ubiquitous or nonspecifically bound native C3 from biologically active C3b fragment, C3b or C are negative with this clone. The antibody inhibits the anaphylactic function of C3a: the capacity of C3a to induce serotonin release from thrombocytes in vitro is inhibited; similarly, treatment of C3a with the antibody inhibits the C3a-induced skin reaction in vivo. Species cross-reactivity: Guinea pig and rabbit C3/C3a.
Applications
- IHC
- IHC - Frozen
- Western blot
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Usage
Suitable for use in Immunohistochemistry (frozen sections) and Western blotting. The antibody is, in combination with antibodies to the C3b fragment, an excellent control for distinguishing ubiquitous or nonspecifically bound native C3 from the biologically active C3 fragment. Nonactivated C3 still bears the C3a determinant, whereas activated C3 (C3b, C3d) is C3a-negative. It is therefore useful for immunopathological assays (demonstration of C3 deposition in biopsies) and for C3a assays research purposes.
Presentation
Lyophilized from PBS, pH 7.4, 0.5% BSA, 0.9% sodium azide
Reconstitution
Reconstitute with 1.0 ml sterile ddH2O
Storage
Lyophilized and resuspended forms: store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee