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Anti-COLQ Antibody LS-C155496

Ordering

Wt. Vol. Conc. Price
- 100 µl 1 mg/ml $335

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

COLQ Antibody LS-C155496 Images

[click image for a larger image and more details]
Immunohistochemistry
IHC of paraffin-embedded Colon ca, using COLQ antibody at 1:500 dilution.
Western Blot
Sample (30 ug of whole cell lysate) A: HepG2 10% SDS PAGE COLQ antibody diluted at 1:1000

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Rabbit Polyclonal (IgG) to Human COLQ
Human
IHC - Paraffin, Western blot
Unconjugated

Details

Human COLQ
Rabbit
Human (tested or 100% immunogen sequence identity)
IgG Polyclonal
Immunoaffinity purified

Applications

IHC - Paraffin (1:100 - 1:1000), Western blot (1:500 - 1:3000)

Specificity and Use

COLQ antibody was raised against recombinant fragment corresponding to a region within amino acids 248 and 455 of COLQ (SwissProt Q9Y215). Percent identity by BLAST analysis: Human (100%); Mouse, Bovine (87%).
Human COLQ
Suggested starting dilutions are as follows: Western blot: 1:500-1:3000, IHC-Paraffin: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10 mM Citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Packaging

0.1 M Tris-glycine, pH 7, 20% Glycerol, 0.01% Thimerosal
Aliquot and freeze at -20° C. Avoid freeze-thaw cycles.
For research use only.

About COLQ

Q9Y215 NM_005677 NP_005668.2

COLQ Antibody, AChE Q subunit Antibody, EAD Antibody


Immunohistochemistry

Immunohistochemistry
IHC of paraffin-embedded Colon ca, using COLQ antibody at 1:500 dilution.

Western Blot

Western Blot
Sample (30 ug of whole cell lysate) A: HepG2 10% SDS PAGE COLQ antibody diluted at 1:1000

Requested From: United States
Date Requested: 7/24/2014
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