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Anti-CHRM3 / M3 Antibody (Cytoplasmic Domain) IHC-plus™ LS-A1421

Ordering

Wt. Vol. Conc. Price
50 µg 50 µl 1 mg/ml $395
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CHRM3 / M3 Antibody LS-A1421 Images

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Immunohistochemistry
Anti-CHRM3 / M3 antibody LS-A1421 IHC of human heart.

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Rabbit Polyclonal to Human CHRM3 / M3
Human, Chimpanzee, Gorilla, Gibbon, Monkey
IHC - Paraffin, ELISA
Unconjugated

Details

Human CHRM3 / M3
Rabbit
Human, Chimpanzee, Gorilla, Gibbon, Monkey (tested or 100% immunogen sequence identity)
Orangutan, Bovine, Hamster, Horse, Sheep (at least 90% immunogen sequence identity)
Polyclonal
Immunoaffinity purified

Applications

IHC - Paraffin (10 µg/ml), ELISA

Specificity and Use

CHRM3 / M3 antibody was raised against synthetic 18 amino acid peptide from 3rd cytoplasmic domain of human CHRM3 / M3. Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset (100%); Orangutan, Sheep, Bovine, Hamster, Horse (94%); Elephant, Pig (89%); Mouse, Rat, Dog, Rabbit, Opossum, Guinea pig, Turkey, Chicken, Lizard (83%).
LS-E28269 - Liquid - 50 µg - $100.00
Cytoplasmic Domain
Human CHRM3 / M3. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Immunohistochemistry: LS-A1421 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-A1421 was determined to be 10 ug/ml.

Packaging

PBS, 0.1% sodium azide.
Long term: -70°C; Short term: +4°C
For research use only.

About CHRM3 / M3

P20309 NM_000740 NP_000731.1

CHRM3 Antibody, M3 Antibody, Muscarinic receptor M3 Antibody, Muscarinic M3 receptor Antibody, EGBRS Antibody, HM3 Antibody, M3 muscarinic receptor Antibody

Muscarinic Acetylcholine Receptor 3 (CHRM3) is an Acetylcholine Receptor that controls smooth muscle contraction by mediating phosphoinositide hydrolysis and Ca2+ mobilization. Activation of CHRM3 causes secretion in glandular tissues. Mouse studies suggest that the M3 receptor may play a role in appetite stimulation and food intake.

Immunohistochemistry

Immunohistochemistry
Anti-CHRM3 / M3 antibody LS-A1421 IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
 

Requested From: United States
Date Requested: 7/22/2014
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