CHRM3 / M3 antibody was raised against synthetic 18 amino acid peptide from 3rd cytoplasmic domain of human CHRM3 / M3. Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset (100%); Orangutan, Sheep, Bovine, Hamster, Horse (94%); Elephant, Pig (89%); Mouse, Rat, Dog, Rabbit, Opossum, Guinea pig, Turkey, Chicken, Lizard (83%).
Human CHRM3 / M3. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Immunohistochemistry: LS-A1421 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-A1421 was determined to be 10 ug/ml.
Muscarinic Acetylcholine Receptor 3 (CHRM3) is an Acetylcholine Receptor that controls smooth muscle contraction by mediating phosphoinositide hydrolysis and Ca2+ mobilization. Activation of CHRM3 causes secretion in glandular tissues. Mouse studies suggest that the M3 receptor may play a role in appetite stimulation and food intake.