Target
Human CHKB / CHKL
Synonyms
CHKB | Choline kinase beta | Choline kinase-like | CK | CHETK | CKEKB | CHKL | Choline/ethanolamine kinase | Ethanolamine kinase beta | Ethanolamine kinase | MDCMC | Choline kinase-like protein | EK | EKB
Reactivity
Human, Monkey
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
Peptide with sequence C-KGETLSQEEQRKLEED, from the internal region of the protein sequence according to NP_005189.2.
Specificity
Human CHKB / CHKL. This antibody is expected to recognize isoform a (NP_005189.2) only.
Applications
- Peptide Enzyme-Linked Immunosorbent Assay (1:2000)
Usage
Peptide ELISA: antibody detection limit dilution 1:2000. Western blot: Preliminary experiments gave an approx 38kDa band in Human Skeletal Muscle lysates after 1 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 45.3kDa according to NP_005189.2. The 38kDa band was successfully blocked by incubation with the immunizing peptide.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee