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Anti-CDK9 Antibody IHC-plus™ LS-B354

Ordering

Wt. Vol. Conc. Price
- 50 µl - $395

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

CDK9 Antibody LS-B354 Images

[click image for a larger image and more details]
Immunohistochemistry
Anti-CDK9 antibody IHC of human skin.
ICC
Anti-cdk9 (PITALRE) Antibody - Immunocytochemical Staining.
Western Blot
Anti-cdk9 (PITALRE) Antibody - Western blot.
 

100% Guaranteed 100% Guaranteed Publications Publications (5)
Rabbit Polyclonal to Human CDK9
Human, Mouse, Rat
IHC - Paraffin, ICC, Western blot, Immunoprecipitation, ELISA
Unconjugated

Details

Human CDK9
Rabbit
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Polyclonal
Delipidated and defibrinated

Applications

IHC - Paraffin (1:500), ICC, Western blot (1:500 - 1:3000), Immunoprecipitation, ELISA (1:10000 - 1:50000)

Specificity and Use

CDK9 antibody was raised against synthetic peptide from human CDK9.
Multiple synthetic peptides corresponding to C-terminal and N-terminal domains of the protein coded by the human gene cdk9 (PITALRE).
Immunohistochemistry: LS-B354 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B354 was determined to be 1:500.

Packaging

0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
Store vial at -20 C or below prior to opening. Dilute only prior to immediate use. Aliquot contents and freeze at -20 C or below. Avoid cycles of freezing and thawing.
For research use only.

Publications (5)

1
Chromosomal mapping of members of the cdc2 family of protein kinases, cdk3, cdk6, PISSLRE, and PITALRE, and a cdk inhibitor, p27Kip1, to regions involved in human cancer. Graa X, De Luca A, Sang N, Fu Y, Claudio PP, Rosenblatt J, Morgan DO, Giordano A. Cancer research. 1995 55:1199-205. [PubMed:7882308]
2
PITALRE, a nuclear CDC2-related protein kinase that phosphorylates the retinoblastoma protein in vitro. Graa X, De Luca A, Sang N, Fu Y, Claudio PP, Rosenblatt J, Morgan DO, Giordano A. Proceedings of the National Academy of Sciences of the United States of America. 1994 91:3834-8. [PubMed:8170997] [PMC:PMC43676]
3
Cloning of murine CDK9/PITALRE and its tissue-specific expression in development. Bagella L, MacLachlan TK, Buono RJ, Pisano MM, Giordano A, De Luca A. Journal of cellular physiology. 1998 177:206-13. [PubMed:9766517]
4
Binding of CDK9 to TRAF2. MacLachlan TK, Sang N, De Luca A, Puri PL, Levrero M, Giordano A. Journal of cellular biochemistry. 1998 71:467-78. [PubMed:9827693]
5
CDK9 (PITALRE): a multifunctional cdc2-related kinase. de Falco G, Giordano A. Journal of cellular physiology. 1998 177:501-6. [PubMed:10092203]
more

About CDK9

P50750 NM_001261 NP_001252.1

CDK9 Antibody, C-2k Antibody, CDC2L4 Antibody, CDC2-related kinase Antibody, Cell division protein kinase 9 Antibody, Cyclin-dependent kinase 9 Antibody, CDC2-related protein kinase Antibody, PITALRE Antibody, TAK Antibody, CTK1 Antibody

CDK9, a CDC2/CDK type protein kinase, is a component of the multiprotein complex TAK/P-TEFb, which is an elongation factor for RNA polymerase II-directed transcription. CDK9 associates with cyclin K or T, and this complex phosphorylates the C-terminal domain (CTD) of the holoenzyme. CDK9/cyclin K also has been shown to phosphorylate the retinoblastoma protein (Rb) and myelin basic protein. At least two mRNA transcripts have been reported -- 2.8- and 4.3-kb.

Immunohistochemistry

Immunohistochemistry
Anti-CDK9 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B354 dilution 1:500.

ICC

ICC
Anti-cdk9 (PITALRE) Antibody - Immunocytochemical Staining. Immunocytochemical staining of mouse tissue using anti-cdk9 (PITALRE) antiserum. The staining shows the location of mcdk9/PITALRE protein in developing mouse tissue. Arrows indicate areas of high expression. Panel A: Peroxidase-DAB immunostaining of mcdk9/PITALRE protein in the developing mouse brain in the differentiated region of the medulla oblongata just below the fourth ventricle. Similar staining is shown in Panel B in the dorsal root ganglia. Panel C: Fluorescein immunofluorescence of mcdk9IPITALRE in skeletal muscle. Similar staining is shown in Panel D in cardiac muscle. Sections from each specimen were cut at 5-7 micron, mounted on glass and dried overnight at 37°C. All sections then were deparaffinized in xylene, rehydrated through a graded alcohol series and washed in phosphate-buffered saline (PBS). PBS was used for all subsequent washes and for antiserum dilution. Tissue sections were quenched sequentially in 0.5% hydrogen peroxide and blocked with diluted 10% normal goat anti-rabbit serum. Slides were incubated at 20° C for 1 h with rabbit anti-cdk9 (1:500) dilution, washed, and then reacted with diluted goat anti-rabbit biotinylated antibody for 30 min. All the slides were then reacted with streptavidin-peroxidase conjugate for 30 min at 20° C. Diaminobenzidine was used as the final chromogen and hematoxylin was used as the nuclear counterstain.

Western Blot

Western Blot
Anti-cdk9 (PITALRE) Antibody - Western blot. Anti-cdk9 antibody (LS-B354 1:1500 ) was used for Western blot of 1) PC3 and 2) DU145 prostate cancer cells (50 ug per lane). Bands at the expected MW of 55 and 42 Kda were detected. Personal communication Flavio Rizzolio, Temple University.
 

Requested From: United States
Date Requested: 10/22/2014

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