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Anti-CD47 Antibody (Intracellular Domain) [clone EPR4150(2)] IHC-plus™ LS-B8451

Ordering

Wt. Vol. Conc. Price
- 50 µl - $395

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

CD47 Antibody LS-B8451 Images

[click image for a larger image and more details]
Immunohistochemistry
Anti-CD47 antibody IHC of human prostate, epithelium.
Flow Cytometry
Flow cytometry of permeabilized Jurkat cells using anti-CD47 antibody (red)or a rabbit IgG...
Western Blot
Western blot analysis on (A) Jurkat, (B) HeLa, and (C) K562 cell lysates using anti-CD47 antibody.
 

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Rabbit Monoclonal [clone EPR4150(2)] to Human CD47
Human, Mouse, Rat
IHC - Paraffin, ICC, Western blot, Flow Cytometry
Unconjugated

Details

Human CD47
Rabbit
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Monoclonal [EPR4150(2)]
Tissue culture supernatant

Applications

IHC - Paraffin (1:50), ICC (1:100 - 1:250), Western blot (1:1000 - 1:10000), Flow Cytometry (1:100 - 1:500)

Specificity and Use

CD47 antibody was raised against synthetic peptide
Intracellular Domain
Human CD47. Species cross-reactivity is based on WB analysis.

Packaging

Tris-Glycine-NaCl or PBS, pH 7.2-7.4, 40-50% Glycerol, 0.01% sodium azide, 0.05% BSA
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
For research use only.

About CD47

Q08722 NM_001777 NP_001768.1

CD47 Antibody, CD47 antigen Antibody, CD47 molecule Antibody, Integrin associated protein Antibody, Integrin-associated protein Antibody, Leukocyte surface antigen CD47 Antibody, MER6 Antibody, Protein MER6 Antibody, Rh-related antigen Antibody, OA3 Antibody, CD47 glycoprotein Antibody, IAP Antibody

CD47 is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix. CD47 is also a receptor for the C-terminal cell binding domain of thrombospondin, and it may play a role in membrane transport and signal transduction. CD47 has broad tissue distribution, and is reduced in expression on Rh erythrocytes.


CD47 Antibodies (33)

Target Reactivity Applications Host Clonality Clone Format Epitope Publications
CD47 Sheep Polyclonal Unconjugated Human IHC, WB, Flo, Neut No
Anti-CD47 Antibody LS-C149906
IHC Image
Flo Image
Sheep Polyclonal (IgG) to Human CD47
Human
IHC, Western blot, Flow Cytometry, Neutralization
Unconjugated
CD47 Mouse Monoclonal B6H12.2 Unconjugated Human WB, IP, Flo, Neut No
Anti-CD47 Antibody [clone B6H12.2] LS-C148708
Flo Image
Mouse Monoclonal [clone B6H12.2] (IgG1) to Human CD47
Human
Western blot, Immunoprecipitation, Flow Cytometry, Neutralization
Unconjugated
CD47 Mouse Monoclonal 1/1A4 Unconjugated, Low Endotoxin Human IHC-Fr, IP, Flo, Func No
Anti-CD47 Antibody [clone 1/1A4] LS-C188326
Mouse Monoclonal [clone 1/1A4] (IgG2b) to Human CD47
Human
IHC - Frozen, Immunoprecipitation, Flow Cytometry, Functional Assay
Unconjugated, Low Endotoxin
CD47 Rabbit Polyclonal Unconjugated Human IHC-P, WB aa288-315 No
Anti-CD47 Antibody (aa288-315) LS-C168545
IHC Image
WB Image
Rabbit Polyclonal to Human CD47
Human
IHC - Paraffin, Western blot
Unconjugated
CD47 Mouse Monoclonal BRIC126 Unconjugated Human, Bovine, Dog, Pig, Sheep IHC-Fr, WB, IP, Flo No
Anti-CD47 Antibody [clone BRIC126] LS-C45437
Mouse Monoclonal [clone BRIC126] (IgG2b) to Human CD47
Human, Bovine, Dog, Pig, Sheep
IHC - Frozen, Western blot, Immunoprecipitation, Flow Cytometry
Unconjugated
CD47 Mouse Monoclonal 1/1A4 Unconjugated Human IHC-Fr, IP, Flo No
Anti-CD47 Antibody [clone 1/1A4] LS-C188327
Mouse Monoclonal [clone 1/1A4] (IgG2b) to Human CD47
Human
IHC - Frozen, Immunoprecipitation, Flow Cytometry
Unconjugated
CD47 Mouse Monoclonal BRIC126 Unconjugated Human IHC-Fr, IP, Flo No
Anti-CD47 Antibody [clone BRIC126] LS-C202089
Mouse Monoclonal [clone BRIC126] (IgG2b) to Human CD47
Human
IHC - Frozen, Immunoprecipitation, Flow Cytometry
Unconjugated
CD47 Mouse Monoclonal MEM-122 Unconjugated Monkey, Human IHC-Fr, WB, Flo No
Anti-CD47 Antibody [clone MEM-122] LS-C45820
Mouse Monoclonal [clone MEM-122] (IgM) to Monkey CD47
Monkey, Human
IHC - Frozen, Western blot, Flow Cytometry
Unconjugated
CD47 Goat Polyclonal Unconjugated Mouse IHC-Fr, WB No
Anti-CD47 Antibody LS-C150168
Goat Polyclonal (IgG) to Mouse CD47
Mouse
IHC - Frozen, Western blot
Unconjugated
CD47 Mouse Monoclonal 1/1A4 Unconjugated Human IHC-Fr, IP, Flo, Func No
Anti-CD47 Antibody [clone 1/1A4] LS-C123043
Mouse Monoclonal [clone 1/1A4] (IgG2b) to Human CD47
Human
IHC - Frozen, Immunoprecipitation, Flow Cytometry, Functional Assay
Unconjugated
CD47 Mouse Monoclonal 1/1A4 Unconjugated Human IHC-Fr, IP, Flo No
Anti-CD47 Antibody [clone 1/1A4] LS-C123042
Mouse Monoclonal [clone 1/1A4] (IgG2b) to Human CD47
Human
IHC - Frozen, Immunoprecipitation, Flow Cytometry
Unconjugated
CD47 Mouse Monoclonal 1/1A4 Unconjugated Human IHC-Fr, IP, Flo No
Anti-CD47 Antibody [clone 1/1A4] LS-C123044
Mouse Monoclonal [clone 1/1A4] (IgG2b) to Human CD47
Human
IHC - Frozen, Immunoprecipitation, Flow Cytometry
Unconjugated
CD47 Rabbit Polyclonal Unconjugated Human WB, ELISA aa23-140 No
Anti-CD47 Antibody (aa23-140) LS-C178715
WB Image
Rabbit Polyclonal to Human CD47
Human
Western blot, ELISA
Unconjugated
CD47 Mouse Monoclonal B6H12.2 PE Conjugated Human Flo No
Anti-CD47 Antibody [clone B6H12.2] (PE) LS-C148707
Flo Image
Mouse Monoclonal [clone B6H12.2] (IgG1) to Human CD47
Human
Flow Cytometry
PE Conjugated
CD47 Mouse Monoclonal B6H12.2 FITC Conjugated Human Flo No
Anti-CD47 Antibody [clone B6H12.2] (FITC) LS-C148706
Flo Image
Mouse Monoclonal [clone B6H12.2] (IgG1) to Human CD47
Human
Flow Cytometry
FITC Conjugated
CD47 Rabbit Polyclonal Unconjugated Human, Horse WB N-Terminus No
Anti-CD47 Antibody (N-Terminus) LS-C146371
WB Image
Rabbit Polyclonal to Human CD47
Human, Horse
Western blot
Unconjugated
CD47 Mouse Monoclonal B6H12.2 Unconjugated, Azide-free Human IF, IP, Flo, Block No
Anti-CD47 Antibody [clone B6H12.2] LS-C87684
Mouse Monoclonal [clone B6H12.2] (IgG1,k) to Human CD47
Human
Immunofluorescence, Immunoprecipitation, Flow Cytometry, Blocking
Unconjugated, Azide-free
CD47 Mouse Monoclonal B6H12.2 Unconjugated Human IF, IP, Flo, Block No
Anti-CD47 Antibody [clone B6H12.2] LS-C87683
Mouse Monoclonal [clone B6H12.2] (IgG1,k) to Human CD47
Human
Immunofluorescence, Immunoprecipitation, Flow Cytometry, Blocking
Unconjugated
CD47 Mouse Monoclonal FITC Conjugated Human Flo No
Anti-CD47 Antibody (FITC) LS-C44943
Mouse Monoclonal (IgG2b) to Human CD47
Human
Flow Cytometry
FITC Conjugated
CD47 Mouse Monoclonal MEM-122 FITC Conjugated Monkey, Human, Pig, Primate No
Anti-CD47 Antibody [clone MEM-122] (FITC) LS-C46357
Mouse Monoclonal [clone MEM-122] (IgM) to Monkey CD47
Monkey, Human, Pig, Primate
FITC Conjugated
CD47 Mouse Monoclonal Unconjugated Rat WB, IP, Flo, Func No
Anti-CD47 Antibody LS-C5230
Mouse Monoclonal (IgG1) to Rat CD47
Rat
Western blot, Immunoprecipitation, Flow Cytometry, Functional Assay
Unconjugated
CD47 Mouse Monoclonal Unconjugated Rat WB, IP, Flo No
Anti-CD47 Antibody LS-C5228
Mouse Monoclonal (IgG1) to Rat CD47
Rat
Western blot, Immunoprecipitation, Flow Cytometry
Unconjugated
CD47 Rabbit Polyclonal FITC Conjugated Human WB, ELISA C-Terminus No
Anti-CD47 Antibody (C-Terminus) (FITC) LS-C254072
Rabbit Polyclonal (IgG) to Human CD47
Human
Western blot, ELISA
FITC Conjugated
CD47 Rabbit Polyclonal HRP Conjugated Human WB, ELISA C-Terminus No
Anti-CD47 Antibody (C-Terminus) (HRP) LS-C254073
Rabbit Polyclonal (IgG) to Human CD47
Human
Western blot, ELISA
HRP Conjugated
CD47 Rabbit Polyclonal PE Conjugated Human WB, ELISA C-Terminus No
Anti-CD47 Antibody (C-Terminus) (PE) LS-C254074
Rabbit Polyclonal (IgG) to Human CD47
Human
Western blot, ELISA
PE Conjugated
CD47 Rabbit Polyclonal Biotin Conjugated Human WB, ELISA C-Terminus No
Anti-CD47 Antibody (C-Terminus) (Biotin) LS-C254070
Rabbit Polyclonal (IgG) to Human CD47
Human
Western blot, ELISA
Biotin Conjugated
CD47 Mouse Monoclonal Unconjugated Rat IP, Flo No
Anti-CD47 Antibody LS-C5234
Mouse Monoclonal (IgG1) to Rat CD47
Rat
Immunoprecipitation, Flow Cytometry
Unconjugated
CD47 Rabbit Polyclonal AP Conjugated Human WB, ELISA C-Terminus No
Anti-CD47 Antibody (C-Terminus) (AP) LS-C254068
Rabbit Polyclonal (IgG) to Human CD47
Human
Western blot, ELISA
AP Conjugated
CD47 Rabbit Polyclonal APC Conjugated Human WB, ELISA C-Terminus No
Anti-CD47 Antibody (C-Terminus) (APC) LS-C254069
Rabbit Polyclonal (IgG) to Human CD47
Human
Western blot, ELISA
APC Conjugated
CD47 Mouse Monoclonal PE Conjugated Rat, Human Flo No
Anti-CD47 Antibody (PE) LS-C5233
Mouse Monoclonal (IgG1) to Rat CD47
Rat, Human
Flow Cytometry
PE Conjugated
CD47 Mouse Monoclonal FITC Conjugated Rat, Human Flo No
Anti-CD47 Antibody (FITC) LS-C5232
Mouse Monoclonal (IgG1) to Rat CD47
Rat, Human
Flow Cytometry
FITC Conjugated
CD47 Mouse Monoclonal Biotin Conjugated Rat, Human Flo No
Anti-CD47 Antibody (Biotin) LS-C5231
Mouse Monoclonal (IgG1) to Rat CD47
Rat, Human
Flow Cytometry
Biotin Conjugated
CD47 Rabbit Monoclonal EPR4150(2) Unconjugated Human, Mouse, Rat IHC-P, ICC, WB, Flo Intracellular Domain No
Anti-CD47 Antibody (Intracellular Domain) [clone EPR4150(2)] IHC-plus™ LS-B8451
IHC Image
Flo Image
Rabbit Monoclonal [clone EPR4150(2)] to Human CD47
Human, Mouse, Rat
IHC - Paraffin, ICC, Western blot, Flow Cytometry
Unconjugated

Immunohistochemistry

Immunohistochemistry
Anti-CD47 antibody IHC of human prostate, epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8451 dilution 1:50.

Flow Cytometry

Flow Cytometry
Flow cytometry of permeabilized Jurkat cells using anti-CD47 antibody (red)or a rabbit IgG (negative) (green).

Western Blot

Western Blot
Western blot analysis on (A) Jurkat, (B) HeLa, and (C) K562 cell lysates using anti-CD47 antibody.
 
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Requested From: United States
Date Requested: 4/18/2014
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