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Anti-CD4 Antibody (C-Terminus) IHC-plus™ LS-B3426

Ordering

Wt. Vol. Conc. Price
- 200 µl - $395

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

CD4 Antibody LS-B3426 Images

[click image for a larger image and more details]
Immunohistochemistry
Anti-CD4 antibody IHC of human tonsil.
Flow Cytometry
Flow cytometric analysis of CEM cells using CD4 Antibody (C-term) (bottom histogram) compared to...
Western Blot
Western blot of mouse heart tissue lysates (35 ug/lane).
Western Blot
Western blot analysis of anti-CD4 Antibody (C-term) in mouse heart tissue lysates (35...

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Rabbit Polyclonal to Human CD4
Human, Mouse
IHC - Paraffin, Western blot, Flow Cytometry
Unconjugated

Details

Human CD4
Rabbit
Human, Mouse (tested or 100% immunogen sequence identity)
Polyclonal
Immunoaffinity purified

Applications

IHC - Paraffin (10 µg/ml), Western blot (1:1000), Flow Cytometry (1:10 - 1:50)

Specificity and Use

CD4 antibody was raised against kLH conjugated synthetic peptide selected from the C-terminal region of human CD4.
LS-E3759 - Lyophilized - 100 µg - $145.00
C-Terminus
Cross-reactivity with mouse has been confirmed in WB only. The antibody has not been evaluated for use in IHC with mouse samples.
Immunohistochemistry: LS-B3426 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3426 was determined to be 10 ug/ml.

Packaging

PBS, 0.09% sodium azide
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
For research use only.

About CD4

P01730 NM_000616 NP_000607.1

CD4 Antibody, CD4 antigen Antibody, CD4 receptor Antibody, CD4mut Antibody, CD4 antigen (p55) Antibody, CD4 molecule Antibody

CD4 is a membrane glycoprotein of T lymphocytes that interacts with major histocompatibility complex class II antigenes and is also a receptor for the human immunodeficiency virus. CD4 is expressed not only in T lymphocytes, but also in B cells, macrophages, and granulocytes. It is also expressed in specific regions of the brain.

Immunohistochemistry

Immunohistochemistry
Anti-CD4 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3426 concentration 10 ug/ml.

Flow Cytometry

Flow Cytometry
Flow cytometric analysis of CEM cells using CD4 Antibody (C-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western Blot

Western Blot
Western blot of mouse heart tissue lysates (35 ug/lane).

Western Blot

Western Blot
Western blot analysis of anti-CD4 Antibody (C-term) in mouse heart tissue lysates (35 ug/lane).CD4 (arrow) was detected using the purified Pab.

Requested From: United States
Date Requested: 7/25/2014
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