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Anti-CCND1 / Cyclin D1 Antibody (Ser90) IHC-plus™ LS-B3452

Ordering

Wt. Vol. Conc. Price
50 µg - 0.25 mg/ml $395

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CCND1 / Cyclin D1 Antibody LS-B3452 Images

[click image for a larger image and more details]
Immunohistochemistry
Anti-CCND1 / Cyclin D1 antibody IHC of human testis.
Immunohistochemistry
Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with Cyclin D1...
Immunohistochemistry
Formalin-fixed and paraffin-embedded human Testis tissue reacted with Cyclin D1 Antibody (S90),...
Immunofluorescence
Confocal immunofluorescence of Cyclin D1 Antibody (S90)LS-B3452 with HeLa cell followed by Alexa...
Western Blot
Western blot of mouse lung and bladder tissue lysates (35 ug/lane).
Western Blot
Western blot analysis of anti-Cyclin D1 Antibody (S90) in mouse lung lysates (35 ug/lane).

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Rabbit Polyclonal to Human CCND1 / Cyclin D1
Human, Mouse
IHC - Paraffin, Immunofluorescence, Western blot
Unconjugated

Details

Human CCND1 / Cyclin D1
Rabbit
Human, Mouse (tested or 100% immunogen sequence identity)
Polyclonal
Immunoaffinity purified

Applications

IHC - Paraffin (10 µg/ml), Immunofluorescence (1:10 - 1:50), Western blot (1:1000)

Specificity and Use

CCND1 / Cyclin D1 antibody was raised against kLH conjugated synthetic peptide corresponding to amino acid residues surrounding S90 of human Cyclin D1.
LS-E7012 - Lyophilized - 100 µg - $145.00
Ser90
Immunohistochemistry: LS-B3452 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3452 was determined to be 10 ug/ml. Please note that cross-reactivity with mouse has been confirmed in WB only at this time. The antibody has not yet been evaluated for use in IHC with mouse samples.

Packaging

PBS, 0.09% sodium azide
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
For research use only.

About CCND1 / Cyclin D1

P24385 NM_053056 NP_444284.1

CCND1 Antibody, B-cell lymphoma 1 protein Antibody, BCL-1 oncogene Antibody, B-cell CLL/lymphoma 1 Antibody, BCL-1 Antibody, Cyclin D1 Antibody, G1/S-specific cyclin-D1 Antibody, U21B31 Antibody, PRAD1 Antibody, BCL1 Antibody, D11S287E Antibody, PRAD1 oncogene Antibody

The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event.

Immunohistochemistry

Immunohistochemistry
Anti-CCND1 / Cyclin D1 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3452 concentration 10 ug/ml.

Immunohistochemistry

Immunohistochemistry
Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with Cyclin D1 Antibody (S90), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Immunohistochemistry

Immunohistochemistry
Formalin-fixed and paraffin-embedded human Testis tissue reacted with Cyclin D1 Antibody (S90), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Immunofluorescence

Immunofluorescence
Confocal immunofluorescence of Cyclin D1 Antibody (S90)LS-B3452 with HeLa cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).

Western Blot

Western Blot
Western blot of mouse lung and bladder tissue lysates (35 ug/lane).

Western Blot

Western Blot
Western blot analysis of anti-Cyclin D1 Antibody (S90) in mouse lung lysates (35 ug/lane). Cyclin D1(arrow) was detected using the purified Pab.

Requested From: United States
Date Requested: 7/23/2014
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