Human CCNB1 / Cyclin B1

Rabbit

Human, Chimpanzee, Mouse, Rat, Dog, Hamster (tested or 100% immunogen sequence identity)

IgG Polyclonal

Unconjugated

Affinity purified

Unmodified

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 115-140 of Human Cyclin B1 protein.

pSer126

This antibody is specific for phosphorylated human Cyclin B1 protein at the pS126 residue. BLAST analysis indicates 100 % homology of the immunizing sequence with Cyclin B1 from human. Cross reactivity may occur with Cyclin B1 from rat, dog and chimpanzee as only a single amino acid change was detected within the immunogen sequence from these sources (91% homology). Partial reactivity may also occur with Cyclin B1 from mouse and hamster where two amino acids changes was noted (83% homology). Minimal reactivity is expected with the non-phosphorylated form of the protein.

• Western blot (1:100 - 1:1000)
• Immunoprecipitation (1:100)
• ELISA (1:50000)

This affinity purified antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band - 48 kD in size corresponding to Cyclin B1 by western blotting in the appropriate cell lysate or extract.

0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide

Store vial at -20°C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P14635 NCBI: NM_031966 NP_114172.1