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Anti-C-Kit / CD117 Antibody (Extracellular Domain) IHC-plus™ LS-A9389

Ordering

Wt. Vol. Conc. Price
50 µg 50 µl 1 mg/ml $395
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C-Kit / CD117 Antibody LS-A9389 Images

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Immunohistochemistry
Anti-c-Kit antibody LS-A9389 IHC of human spleen, central artery and lymphocytes within...

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Rabbit Polyclonal to Human C-Kit / CD117
Human, Gorilla
IHC - Paraffin
Unconjugated

Details

Human C-Kit / CD117
Rabbit
Human, Gorilla (tested or 100% immunogen sequence identity)
Gibbon, Monkey (at least 90% immunogen sequence identity)
Polyclonal
Immunoaffinity purified

Applications

IHC - Paraffin (20 µg/ml)

Specificity and Use

C-Kit / CD117 antibody was raised against synthetic 16 amino acid peptide from extracellular domain of human c-Kit. Percent identity with other species by BLAST analysis: Human, Gorilla (100%); Gibbon, Monkey (94%); Marmoset, Mouse, Rat (88%); Hamster, Pig (81%).
LS-E29627 - Liquid - 50 µg - $100.00
Extracellular Domain
Human c-Kit. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Immunohistochemistry: LS-A9389 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-A9389 was determined to be 20 ug/ml.

Packaging

PBS, 0.1% sodium azide.
Long term: -70°C; Short term: +4°C
For research use only.

About C-Kit / CD117

P10721 NM_000222 NP_000213.1

KIT Antibody, C-Kit Antibody, CD117 Antibody, PBT Antibody, Piebald trait Antibody, Piebald trait protein Antibody, Proto-oncogene c-Kit Antibody, SCFR Antibody, Soluble KIT variant 1 Antibody, Tyrosine-protein kinase Kit Antibody, p145 c-kit Antibody, CD117 antigen Antibody

KIT encodes the human homolog of the proto-oncogene c-kit. C-kit was first identified as the cellular homolog of the feline sarcoma viral oncogene v-kit. KIT is a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). Somatic gain-of-function mutations in the extracellular domain, kinase domains 1 and 2 are found in gastrointestinal stromal tumors (GISTs).

Immunohistochemistry

Immunohistochemistry
Anti-c-Kit antibody LS-A9389 IHC of human spleen, central artery and lymphocytes within periarteriolar lymphoid sheaths. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
 

Requested From: United States
Date Requested: 11/1/2014

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