LSBio The Immunohistochemistry Antibody Company
  • Home
  • Antibodies
    • Primary Antibodies
    • Secondary Antibodies
    • IHC-plus Antibodies
    • Isotype Control Antibodies
  • ELISA Kits
    • All Kits
    • Sandwich ELISA Kits
    • EIA Kits
    • Phospho-ELISA Kits
  • Services
    • Immunohistochemistry Studies
    • Tissue Cross-Reactivity Screening
  • Distributors
  • Resources
    • About our Tissue Bank
    • Customer Support
    • How to Buy
    • IHC-plus Protocol
    • LSBio Media
    • Publications
    • Antibody Reports
  • Company
    • About LifeSpan
    • Press Releases
    • Holiday Schedule
    • Contact Us
  • View Cart

Anti-BMI1 / PCGF4 Antibody (aa252-264) IHC-plus™ LS-B346

Ordering

Wt. Vol. Conc. Price
50 µg - 0.91 mg/ml $395

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

BMI1 / PCGF4 Antibody LS-B346 Images

[click image for a larger image and more details]
Immunohistochemistry
Anti-BMI-1 antibody IHC of human skeletal muscle.
Immunofluorescence
Anti-Bmi1 Antibody - Immunofluorescence Microscopy.
Western Blot
Anti-Bmi1 Antibody - Western Blot.
 

100% Guaranteed 100% Guaranteed
Goat Polyclonal to Human BMI1 / PCGF4
Human
IHC - Paraffin, ICC, Immunofluorescence, Western blot, ELISA
Unconjugated

Details

Human BMI1 / PCGF4
Goat
Human (tested or 100% immunogen sequence identity)
Polyclonal
Immunoaffinity purified

Applications

IHC - Paraffin (2.5 µg/ml), ICC, Immunofluorescence, Western blot (1:500 - 1:3000), ELISA (1:5000 - 1:30000)

Specificity and Use

BMI1 / PCGF4 antibody was raised against synthetic peptide from human BMI1.
aa252-264
Amino acids 252-264 of human Bmi1 protein.
Immunohistochemistry: LS-B346 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B346 was determined to be 2.5 ug/ml.

Packaging

0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. Store undiluted.
For research use only.

About BMI1 / PCGF4

P35226 NM_005180 NP_005171.4

BMI1 Antibody, BMI-1 Antibody, Flvi-2/bmi-1 Antibody, FLVI2/BMI1 Antibody, PCGF4 Antibody, Polycomb group protein Bmi1 Antibody, Polycomb complex protein BMI-1 Antibody, Dna-binding protein bmi-1 Antibody, Polycomb group ring finger 4 Antibody, RING finger protein 51 Antibody, RNF51 Antibody


Immunohistochemistry

Immunohistochemistry
Anti-BMI-1 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B346 concentration 5 ug/ml.

Immunofluorescence

Immunofluorescence
Anti-Bmi1 Antibody - Immunofluorescence Microscopy. Immunofluorescence using affinity purified goat anti-Bmi1 shows nuclear staining (green) of methanol fixed (100%, 5 min) HepG2 cells. The cells were blocked and permeabilized in 1%BSA / 10% normal donkey serum / 0.3 M glycine in 0.1% PBS-Tween for 1h prior to incubation with the primary antibody (1:200 dilution) overnight at +4°C and detected with a 488nm fluorescent dye conjugated secondary Ab. Cell nuclei are stained with DAPI (blue) and plasma membranes are stained with WGA (red).

Western Blot

Western Blot
Anti-Bmi1 Antibody - Western Blot. Western blot of Affinity Purified anti-Bmi1 antibody shows detection of a band ~37 kD corresponding to human Bmi1 (arrowhead). Approximately 20 ug of a U2OS whole cell lysate (bone osteosarcoma) was separated by 4-20% SDS-PAGE and transferred onto nitrocellulose. After blocking in PBS containing 5% nonfat dry milk, the membrane was probed overnight at 4C with the primary antibody diluted to 1:1000 in PBS containing 1% nonfat dry milk. The membrane was washed and reacted with a 1:20000 dilution of IRDye800 conjugated Rb-a-Goat IgG [H&L] MX ( for 45 min at room temperature. IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
 

Requested From: United States
Date Requested: 8/20/2014

Get Social With Us! Follow us on Facebook Follow us on Google+ Follow us on LinkedIn
Copyright © 2014 LifeSpan BioSciences, Inc. All Rights Reserved