Human, Mouse (tested or 100% immunogen sequence identity)
Monkey, Rat, Bovine (at least 90% immunogen sequence identity)
ICC (1:100 - 1:1000), Western blot (1:1000 - 1:10000)
Specificity and Use
ARA9 / AIP antibody was raised against recombinant fragment corresponding to a region within amino acids 1 and 330 of ARA9 (SwissProt O00170). Percent identity by BLAST analysis: Human (100%); Monkey (97%); Rat (93%); Bovine (92%).
Human ARA9 / AIP
0.1 M Tris-glycine, pH 7.0, 10% glycerol, 0.01% Thimerosal
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Immunofluorescence of paraformaldehyde-fixed HeLa using ARA9 antibody at 1:200 dilution.
ARA9 antibody [N1C3] detects ARA9 protein by Western blot analysis. A. 30 ug HeLa whole cell lysate/extract. B. 30 ug Molt-4 whole cell lysate/extract. C. 30 ug Raji whole cell lysate/extract. 10 % SDS-PAGE. ARA9 antibody [N1C3] dilution:1:5000
Sample (50 ug of whole cell lysate). A: Mouse brain. 10% SDS PAGE. ARA9 / AIP antibody diluted at 1:1000.
Requested From: United States
Date Requested: 4/16/2014