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Anti-AKT1 / AKT Antibody (phospho-Ser473) IHC-plus™ LS-B1183

Ordering

Wt. Vol. Conc. Price
50 µg - 1 mg/ml $395

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

AKT1 / AKT Antibody LS-B1183 Images

[click image for a larger image and more details]
Immunohistochemistry
Anti-AKT1 antibody IHC of human skeletal muscle.
Immunohistochemistry
Anti-AKT1 antibody IHC of human tonsil.
Immunohistochemistry
Immunohistochemistry of Rabbit Anti-Akt pS473 antibody.
Immunofluorescence
Anti-AKT pS473 Antibody - Immunofluorescence Microscopy.
Western Blot
Anti-AKT pS473 Antibody - Western Blot.
 

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Rabbit Polyclonal to Human AKT1 / AKT
Human, Mouse, Rat
IHC - Paraffin, ICC, Immunofluorescence, Western blot, ELISA
Unconjugated

Details

Human AKT1 / AKT
Rabbit
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Polyclonal
Immunoaffinity purified

Applications

IHC - Paraffin (5 µg/ml), ICC, Immunofluorescence, Western blot (1:200 - 1:1000), ELISA (1:15000 - 1:60000)

Specificity and Use

AKT1 / AKT antibody was raised against synthetic peptide from human AKT1.
pSer473
The C-terminus aa 460-480 of human, mouse, rat and chicken AKT proteins conjugated to KLH.
Immunohistochemistry: LS-B1183 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1183 was determined to be 5 ug/ml.

Packaging

0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
+4°C or -20°C, Avoid repeated freezing and thawing.
For research use only.

About AKT1 / AKT

P31749 NM_005163 NP_005154.2

AKT1 Antibody, AKT Antibody, AKT-1 Antibody, C-AKT Antibody, Pan-AKT Antibody, PKB alpha Antibody, PKBalpha Antibody, Proto-oncogene c-Akt Antibody, Protein kinase B Antibody, Rac protein kinase alpha Antibody, PRKBA Antibody, Protein kinase B alpha Antibody, RAC-PK-alpha Antibody, PKB Antibody, PKB-ALPHA Antibody, RAC Antibody, RAC-ALPHA Antibody

AKT1 encodes a RAC/AKT -type protein kinase that contains a N-terminal pleckstrin-homology (PH) domain and a central catalytic domain closely related to both cAMP-dependent protein kinase and protein kinase C. The protein is a member of PI3K-mediated signaling pathways associated with the regulation of proliferation, survival, protein synthesis, and metabolism.

Immunohistochemistry

Immunohistochemistry
Anti-AKT1 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1183 concentration 5 ug/ml.

Immunohistochemistry

Immunohistochemistry
Anti-AKT1 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1183 concentration 5 ug/ml.

Immunohistochemistry

Immunohistochemistry
Immunohistochemistry of Rabbit Anti-Akt pS473 antibody. Tissue: human breast carcinoma. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Akt pS473 antibody at 100 dilution for 1 h at RT. Secondary antibody: Dako's Techmate streptavidin-biotin reagents at 1:10000 for 45 min at RT. Localization: Akt pS473 is nuclear and occasionally cytoplasmic.

Immunofluorescence

Immunofluorescence
Anti-AKT pS473 Antibody - Immunofluorescence Microscopy. Confocal image using Rabbit anti-AKT pS473 at a 1:40 dilution to detect phosphorylated AKT (green) by immunofluorescence microscopy in cardiomyocytes infected with adenovirus expressing with wild-type AKT in conjunction with a Texas-red conjugated phalloidin (red) to label filamentous actin.

Western Blot

Western Blot
Anti-AKT pS473 Antibody - Western Blot. Rabbit anti-AKT pS473 was used at a 1:200 dilution to detect phosphorylated AKT by Western blot. A nuclear extract from cells infected with adenovirus expressing nuclear-targeted AKT kinase was used.
 

Requested From: United States
Date Requested: 8/23/2014

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