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Anti-ABCB1 / MDR1 / P Glycoprotein Antibody (Cytoplasmic Domain) IHC-plus™ LS-A9403

Ordering

Wt. Vol. Conc. Price
50 µg 72 µl 0.7 mg/ml $395
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ABCB1 / MDR1 / P Glycoprotein Antibody LS-A9403 Images

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Immunohistochemistry
Anti-ABCB1 / MDR1 antibody LS-A9403 IHC of human adrenal, cortex.

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Rabbit Polyclonal to Human ABCB1 / MDR1 / P Glycoprotein
Human, Gorilla, Gibbon
IHC - Paraffin
Unconjugated

Details

Human ABCB1 / MDR1 / P Glycoprotein
Rabbit
Human, Gorilla, Gibbon (tested or 100% immunogen sequence identity)
Monkey (at least 90% immunogen sequence identity)
Polyclonal
Immunoaffinity purified

Applications

IHC - Paraffin (20 µg/ml)

Specificity and Use

ABCB1 / MDR1 / P Glycoprotein antibody was raised against synthetic 17 amino acid peptide from cytoplasmic domain of human ABCB1 / MDR1. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon (100%); Monkey (94%); Mouse, Hamster (82%).
LS-E29637 - Liquid - 50 µg - $100.00
Cytoplasmic Domain
Human ABCB1 / MDR1. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Immunohistochemistry: LS-A9403 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-A9403 was determined to be 20 ug/ml.

Packaging

PBS, 0.1% sodium azide.
Long term: -70°C; Short term: +4°C
For research use only.

About ABCB1 / MDR1 / P Glycoprotein

P08183 NM_000927 NP_000918.2

ABCB1 Antibody, ABC20 Antibody, Abcb1b Antibody, CLCS Antibody, Colchicin sensitivity Antibody, CD243 Antibody, Doxorubicin resistance Antibody, IBD13 Antibody, gp170 Antibody, MDR1 Antibody, Multidrug resistance protein 1 Antibody, P glycoprotein Antibody, P-glycoprotein 1 Antibody, P-GP Antibody, CD243 antigen Antibody, PGY1 Antibody

ABCB1/MDR1/P Glycoprotein is an ATP dependent transporter that mediates efflux of chemotherapeutic agents from the intracellular milieu. P Glycoprotein has also been associated with volume-regulated chloride channel activity and basal to apical transendothelial migration of mononuclear phagocytes.

Immunohistochemistry

Immunohistochemistry
Anti-ABCB1 / MDR1 antibody LS-A9403 IHC of human adrenal, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
 

Requested From: United States
Date Requested: 9/1/2014

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