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Catalog Number Size Price
LS-C359268-0.1 0.1 ml (1 mg/ml) $386 
ACTB / Beta Actin Antibody - Immunocytochemistry/Immunofluorescence: Beta Actin Antibody (8H10D10) - Confocal immunofluorescence analysis of SK-BR-3 (left) and A549 (right) cells using anti-Beta Actin mAb (red, the secondary Ab is Cy3-Goat anti mouse IgG). Blue: DRAQ5 fluorescent DNA dye.
ACTB / Beta Actin Antibody - Western Blot: beta-Actin Antibody (8H10D10) - Western blot shows a specific band for Beta Actin in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
ACTB / Beta Actin Antibody - Western Blot: Beta Actin Antibody (8H10D10) - Western blot analysis using anti-Beta Actin mAb against NIH/3T3 (1), Jurkat (2), HeLa (3), CHO (4), PC12 (5), HEK293 (6), COS (7), A549 (8) and MCF-7 (9) cell lysates.
ACTB / Beta Actin Antibody - Western Blot: Beta Actin Antibody (8H10D10) - Analysis of Beta Actin expression in 1) HeLa 2) HepG2 and 3) Cos7 whole cell lysates.
ACTB / Beta Actin Antibody - Flow Cytometry: Beta Actin Antibody (8H10D10) - Flow cytometric analysis of MCF-7 cells using anti-Beta Actin mAb (right) and negative control (left).
ACTB / Beta Actin Antibody - Immunocytochemistry/Immunofluorescence: Beta Actin Antibody (8H10D10) - Confocal immunofluorescence analysis of SK-BR-3 (left) and A549 (right) cells using anti-Beta Actin mAb (red, the secondary Ab is Cy3-Goat anti mouse IgG). Blue: DRAQ5 fluorescent DNA dye.
ACTB / Beta Actin Antibody - Western Blot: beta-Actin Antibody (8H10D10) - Western blot shows a specific band for Beta Actin in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
ACTB / Beta Actin Antibody - Western Blot: Beta Actin Antibody (8H10D10) - Western blot analysis using anti-Beta Actin mAb against NIH/3T3 (1), Jurkat (2), HeLa (3), CHO (4), PC12 (5), HEK293 (6), COS (7), A549 (8) and MCF-7 (9) cell lysates.
ACTB / Beta Actin Antibody - Western Blot: Beta Actin Antibody (8H10D10) - Analysis of Beta Actin expression in 1) HeLa 2) HepG2 and 3) Cos7 whole cell lysates.
ACTB / Beta Actin Antibody - Flow Cytometry: Beta Actin Antibody (8H10D10) - Flow cytometric analysis of MCF-7 cells using anti-Beta Actin mAb (right) and negative control (left).
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Monoclonal Mouse anti‑Human ACTB / Beta Actin Antibody (clone 8H10D10, N‑Terminus, IF, WB) LS‑C359268

Monoclonal Mouse anti‑Human ACTB / Beta Actin Antibody (clone 8H10D10, N‑Terminus, IF, WB) LS‑C359268

Antibody:
ACTB / Beta Actin Mouse anti-Human Monoclonal (N-Terminus) (8H10D10) Antibody
Application:
ICC, IF, WB, Flo, ELISA
Reactivity:
Human, Mouse, Rat, Hamster-Chinese, Primate
Format:
Unconjugated, Unmodified
Price
Catalog Number
$386
LS-C359268-0.1
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
ACTB / Beta Actin Mouse anti-Human Monoclonal (N-Terminus) (8H10D10) Antibody
Application:
ICC, IF, WB, Flo, ELISA
Reactivity:
Human, Mouse, Rat, Hamster-Chinese, Primate
Format:
Unconjugated, Unmodified

Specifications

Description
Beta Actin antibody LS-C359268 is an unconjugated mouse monoclonal antibody to Beta Actin (ACTB) (N-Terminus) from human. It is reactive with human, mouse, rat and other species. Validated for ELISA, Flow, ICC, IF and WB.
Target
Human ACTB / Beta Actin
Synonyms
ACTB | Actin, cytoplasmic 1 | Beta-actin | Beta actin | BRWS1 | Actin, beta | Beta cytoskeletal actin | PS1TP5-binding protein 1 | PS1TP5BP1
Host
Mouse
Reactivity
Human, Mouse, Rat, Hamster-Chinese, Primate (tested or 100% immunogen sequence identity)
Clonality
IgG2b Monoclonal
Clone
8H10D10
Conjugations
Unconjugated
Purification
Ammonium sulfate precipitation
Modifications
Unmodified
Immunogen
Synthetic peptide corresponding to N-Terminus residues of human Beta Actin, conjugated to KLH. [UniProt# P60709]
Epitope
N-Terminus
Specificity
Human ACTB / Beta Actin. Human ACTB / Beta Actin
Applications
  • ICC (1:200 - 1:1000)
  • Immunofluorescence (1:200 - 1:1000)
  • Western blot
  • Flow Cytometry (1:200 - 1:400)
  • ELISA (1:10000)
Usage
This Beta Actin (8H10D10) antibody is useful in Western blot, Flow Cytometry, Immunocytochemistry/Immunofluorescence and ELISA.In Simple Western only 10-15 uL of the recommended dilution is used per data point.
Presentation
PBS, 0.03% Sodium Azide
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ACTB / Beta Actin
P60709 NM_001101 NP_001092.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/28/2024