Measured by its binding ability in a functional ELISA. Immobilized zebrafish EFNB2A-His at 10 µg/ml (100 µl/well) can bind human EphB4-Fc, The EC50 of human EphB4-Fc is 15.5-36.3 ng/ml.

Zebrafish efnb2a Protein (Recombinant His,C-terminus)

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Expression System	Human Embryonic Kidney 293
Format:	Lyophilized

$582.00each

Catalog Number: LS-G49083-200

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Overview

Description: efnb2a Protein LS-G49083 is a Recombinant Zebrafish efnb2a produced in HEK 293 Cells Met1-Ala222 with His,C-terminus tag(s). It is biologically active and is low in endotoxin; Less than 1.0 EU/µg protein (determined by LAL method). For Research Use Only
Category: Proteins & Peptides > Proteins > Recombinant Proteins
Origin Species: Zf
SwissProt: O73874

Protein

Expression System: HEK293
Fusion Tag: 6×His
Molecular Weight: The recombinant zebrafish EFNB2A consists of 209 amino acids and has a calculated molecular mass of 23.3 kDa. It migrates as an 35-40 kDa band in SDS-PAGE under reducing conditions.
Protein Type: Recombinant
Amino Acid Sequence

Format

Purity: >95%
Conjugate/Label: Unconj
Endotoxin Level: < 1.0 EU/µg
Format: Lyophilized
Formulation: Lyophilized from sterile PBS, pH 7.4, with 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Purity Measurement Method: SDS-PAGE