Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
ZEB1 / AREB6 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G124741-100
Catalog Number: LS-G124741
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human AREB6(ZEB1) (NM_001128128), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- AREB6 | BZP | FECD6 | NIL-2-A zinc finger protein | TCF-8 | Negative regulator of IL2 | NIL2A | ZEB | ZEB1 | ZFHEP | DELTAEF1 | NIL-2-A | PPCD3 | TCF8 | Transcription factor 8 | ZFHX1A
- Origin Species
- Hu
- NCBI GenBank
- NM_030751
- SwissProt
- P37275
Protein
- Expression System
- HEK293
- Fusion Tag
- FLAG, Myc
- Molecular Weight
- 121.9 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MKVTNYNTVV ETNSDSDDED KLHIVEEESV TDAADCEGVP EDDLPTDQTV LPGRSSEREG61NAKNCWEDDT GKEGQEILGP EAQADEAGCT VKDDECESDA ENEQNHDPNV EEFLQQQDTA121VIFPEAPEED QRQGTPEASG HDENGTPDAF SQLLTCPYCD RGYKRFTSLK EHIKYRHEKN181EDNFSCSLCS YTFAYRTQLE RHMTSHKSGR DQRHVTQSGC NRKFKCTECG KAFKYKHHLK241EHLRIHSGEK PYECPNCKKR FSHSGSYSSH ISSKKCISLI PVNGRPRTGL KTSQCSSPSL301SASPGSPTRP QIRQKIENKP LQEQLSVNQI KTEPVDYEFK PIVVASGINC STPLQNGVFT361GGGPLQATSS PQGMVQAVVL PTVGLVSPIS INLSDIQNVL KVAVDGNVIR QVLENNQANL421ASKEQETINA SPIQQGGHSV ISAISLPLVD QDGTTKIIIN YSLEQPSQLQ VVPQNLKKEN481PVATNSCKSE KLPEDLTVKS EKDKSFEGGV NDSTCLLCDD CPGDINALPE LKHYDLKQPT541QPPPLPAAEA EKPESSVSSA TGDGNLSPSQ PPLKNLLSLL KAYYALNAQP SAEELSKIAD601SVNLPLDVVK KWFEKMQAGQ ISVQSSEPSS PEPGKVNIPA KNNDQPQSAN ANEPQDSTVN661LQSPLKMTNS PVLPVGSTTN GSRSSTPSPS PLNLSSSRNT QGYLYTAEGA QEEPQVEPLD721LSLPKQQGEL LERSTITSVY QNSVYSVQEE PLNLSCAKKE PQKDSCVTDS EPVVNVIPPS781ANPINIAIPT VTAQLPTIVA IADQNSVPCL RALAANKQTI LIPQVAYTYS TTVSPAVQEP841PLKVIQPNGN QDERQDTSSE GVSNVEDQND SDSTPPKKKM RKTENGMYAC DLCDKIFQKS901SSLLRHKYEH TGKRPHECGI CKKAFKHKHH LIEHMRLHSG EKPYQCDKCG KRFSHSGSYS961QHMNHRYSYC KREAEERDST EQEEAGPEIL SNEHVGARAS PSQGDSDERE SLTREEDEDS1021EKEEEEEDKE MEELQEEKEC EKPQGDEEEE EEEEEVEEEE VEEAENEGEE AKTEGLMKDD1081RAESQASSLG QKVGESSEQV SEEKTNEATRTRPLEQKLISEEDLAANDILDYKDDDDKV
1108 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconj
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.