Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
UBASH3A / CLIP4 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G122735-100
Catalog Number: LS-G122735
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human UBASH3A (NM_001001895), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- STS2 | TULA-1 | STS-2 | T-cell ubiquitin ligand 1 | TULA | UBASH3A | Cbl-interacting protein 4
- Origin Species
- Hu
- NCBI GenBank
- NM_018961
- SwissProt
- P57075
Protein
- Expression System
- HEK293
- Fusion Tag
- FLAG, Myc
- Molecular Weight
- 69.6 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MAAGETQLYA KVSNKLKGRS SPSLLEPFLA MGFPVHTALK ALAATGRKTA EEALAWLHDH61CNDHSLDDPI PQEYALFLCP TGPLLEKLQE FWRESKRQCA KNRAHEVFPH VTLCDFFTCE121DQKVECLYEA LKRAGDRLLG SFPTAVPLAL HSSISYLGFF VSGSPADVIR EFAMTFATEA181SLLADCSVKP YTKQLHLTLA HKFYPHHQRT LEQLARAIPL GHSCQWTAAL YSRDMRFVHY241QTLRALFQYK PQNVDELTLS PGDYIFVDPT QQDEASEGWV IGISQRTGCR GFLPENYTDR301ASESDTWVKH RMYTFSLATD LNSRKDGEAS SRCSGEFLPQ TARSLSSLQA LQATIARKSV361LVVRHGERVD QIFGKAWLQQ CSTPDGKYYR PDLNFPCSLP RRSRGIKDFE NDPPLSSCGI421FQSRIAGDAL LDSGIRISSV FASPALRCVQ TAKLILEELK LEKKIKIRVE PGIFEWTKWE481AGKTTPTLMS LEELKEANFN IDTDYRPAFP LSALMPAESY QEYMDRCTAS MVQIVNTCPQ541DTGVILIVSH GSTLDSCTRP LLGLPPRECG DFAQLVRKIP SLGMCFCEEN KEEGKWELVN601PPVKTLTHGA NAVFNWRNWI SGNTRTRPLEQKLISEEDLAANDILDYKDDDDKV
623 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconj
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.