Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
TRAIL-R4 / DCR2 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G100142-100
Catalog Number: LS-G100142
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human DcR2(TNFRSF10D) (NM_003840), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CD264 | Decoy receptors 2 | DCR2 | TNFRSF10D | TRAILR4 | TRAIL-R4 | TRUNDD | CD264 antigen | Decoy receptor 2 | TRAIL receptor 4
- Origin Species
- Human
- NCBI GenBank
- NM_003840
- SwissProt
- Q9UBN6
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 41.7 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MGLWGQSVPT ASSARAGRYP GARTASGTRP WLLDPKILKF VVFIVAVLLP VRVDSATIPR61QDEVPQQTVA PQQQRRSLKE EECPAGSHRS EYTGACNPCT EGVDYTIASN NLPSCLLCTV121CKSGQTNKSS CTTTRDTVCQ CEKGSFQDKN SPEMCRTCRT GCPRGMVKVS NCTPRSDIKC181KNESAASSTG KTPAAEETVT TILGMLASPY HYLIIIVVLV IILAVVVVGF SCRKKFISYL241KGICSGGGGG PERVHRVLFR RRSCPSRVPG AEDNARNETL SNRYLQPTQV SEQEIQGQEL301AELTGVTVES PEEPQRLLEQ AEAEGCQRRR LLVPVNDADS ADISTLLDAS ATLEEGHAKE361TIQDQLVGSE KLFYEEDEAG SATSCLTRTRPLEQKLISEEDLAANDILDYKDDDDKV
386 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.