Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
TNFRSF14 / CD270 / HVEM Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G100138-100
Catalog Number: LS-G100138
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TNFRSF14 (NM_003820), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CD270 | CD270 antigen | CD40-like protein | Herpesvirus entry mediator | Herpesvirus entry mediator A | HVEM | LIGHTR | Herpes virus entry mediator A | TR2 | ATAR | HVEA | TNFRSF14
- Origin Species
- Human
- NCBI GenBank
- NM_003820
- SwissProt
- Q92956
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 30.42 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MEPPGDWGPP PWRSTPRTDV LRLVLYLTFL GAPCYAPALP SCKEDEYPVG SECCPKCSPG61YRVKEACGEL TGTVCEPCPP GTYIAHLNGL SKCLQCQMCD PAMGLRASRN CSRTENAVCG121CSPGHFCIVQ DGDHCAACRA YATSSPGQRV QKGGTESQDT LCQNCPPGTF SPNGTLEECQ181HQTKCSWLVT KAGAGTSSSH WVWWFLSGSL VIVIVCSTVG LIICVKRRKP RGDVVKVIVS241VQRKRQEAEG EATVIEALQA PPDVTTVAVE ETIPSFTGRS PNHTRTRPLE QKLISEEDLA301ANDILDYKDD DDKV
314 aa
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.