Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
TIP48 / RUVBL2 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G100878-100
Catalog Number: LS-G100878
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human RUVBL2(RUVBL2) (NM_006666), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- 48 kDa TBP-interacting protein | ECP-51 | ECP51 | INO80J | INO80 complex subunit J | REPTIN | Reptin52 | RuvB-like 2 (E. coli) | RUVBL2 | RuvB-like 2 | TIP49B | TIP48 | RuvB (E coli homolog)-like 2 | RVB2 | CGI-46 | Repressing pontin 52 | Reptin 52 | Reptin52 protein | TAP54-beta | TBP-interacting protein | 48-KD | TIH2
- Origin Species
- Human
- NCBI GenBank
- NM_006666
- SwissProt
- Q9Y230
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 51 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MATVTATTKV PEIRDVTRIE RIGAHSHIRG LGLDDALEPR QASQGMVGQL AARRAAGVVL61EMIREGKIAG RAVLIAGQPG TGKTAIAMGM AQALGPDTPF TAIAGSEIFS LEMSKTEALT121QAFRRSIGVR IKEETEIIEG EVVEIQIDRP ATGTGSKVGK LTLKTTEMET IYDLGTKMIE181SLTKDKVQAG DVITIDKATG KISKLGRSFT RARDYDAMGS QTKFVQCPDG ELQKRKEVVH241TVSLHEIDVI NSRTQGFLAL FSGDTGEIKS EVREQINAKV AEWREEGKAE IIPGVLFIDE301VHMLDIESFS FLNRALESDM APVLIMATNR GITRIRGTSY QSPHGIPIDL LDRLLIVSTT361PYSEKDTKQI LRIRCEEEDV EMSEDAYTVL TRIGLETSLR YAIQLITAAS LVCRKRKGTE421VQVDDIKRVY SLFLDESRST QYMKEYQDAF LFNELKGETM DTSTRTRPLE QKLISEEDLA481ANDILDYKDD DDKV
494 aa
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.