Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
TH / Tyrosine Hydroxylase Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G123248-100
Catalog Number: LS-G123248
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Tyrosine Hydroxylase(TH) (NM_000360), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- DYT14 | Dystonia 14 | TH | TYH | Tyrosine 3-hydroxylase | Tyrosine hydroxylase | DYT5b | Tyrosine 3-monooxygenase
- Origin Species
- Human
- NCBI GenBank
- NM_000360
- SwissProt
- P07101
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 55.4 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MPTPDATTPQ AKGFRRAVSE LDAKQAEAIM SPRFIGRRQS LIEDARKERE AAVAAAAAAV61PSEPGDPLEA VAFEEKEGKA MLNLLFSPRA TKPSALSRAV KVFETFEAKI HHLETRPAQR121PRAGGPHLEY FVRLEVRRGD LAALLSGVRQ VSEDVRSPAG PKVPWFPRKV SELDKCHHLV181TKFDPDLDLD HPGFSDQVYR QRRKLIAEIA FQYRHGDPIP RVEYTAEEIA TWKEVYTTLK241GLYATHACGE HLEAFALLER FSGYREDNIP QLEDVSRFLK ERTGFQLRPV AGLLSARDFL301ASLAFRVFQC TQYIRHASSP MHSPEPDCCH ELLGHVPMLA DRTFAQFSQD IGLASLGASD361EEIEKLSTLY WFTVEFGLCK QNGEVKAYGA GLLSSYGELL HCLSEEPEIR AFDPEAAAVQ421PYQDQTYQSV YFVSESFSDA KDKLRSYASR IQRPFSVKFD PYTLAIDVLD SPQAVRRSLE481GVQDELDTLA HALSAIGSGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
497 aa + 34 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.