SREBF1 / SREBP-1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G122338-100
Catalog Number: LS-G122338
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SREBP1(SREBF1) (NM_001005291), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- BHLHd1 | SREBP-1 | SREBP-1c | SREBF1 | SREBP1
- Origin Species
- Human
- NCBI GenBank
- NM_004176
- SwissProt
- P36956
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 124.5 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MDEPPFSEAA LEQALGEPCD LDAALLTDIE GEVGAGRGRA NGLDAPRAGA DRGAMDCTFE61DMLQLINNQD SDFPGLFDPP YAGSGAGGTD PASPDTSSPG SLSPPPATLS SSLEAFLSGP121QAAPSPLSPP QPAPTPLKMY PSMPAFSPGP GIKEESVPLS ILQTPTPQPL PGALLPQSFP181APAPPQFSST PVLGYPSPPG GFSTGSPPGN TQQPLPGLPL ASPPGVPPVS LHTQVQSVVP241QQLLTVTAAP TAAPVTTTVT SQIQQVPVLL QPHFIKADSL LLTAMKTDGA TVKAAGLSPL301VSGTTVQTGP LPTLVSGGTI LATVPLVVDA EKLPINRLAA GSKAPASAQS RGEKRTAHNA361IEKRYRSSIN DKIIELKDLV VGTEAKLNKS AVLRKAIDYI RFLQHSNQKL KQENLSLRTA421VHKSKSLKDL VSACGSGGNT DVLMEGVKTE VEDTLTPPPS DAGSPFQSSP LSLGSRGSGS481GGSGSDSEPD SPVFEDSKAK PEQRPSLHSR GMLDRSRLAL CTLVFLCLSC NPLASLLGAR541GLPSPSDTTS VYHSPGRNVL GTESRDGPGW AQWLLPPVVW LLNGLLVLVS LVLLFVYGEP601VTRPHSGPAV YFWRHRKQAD LDLARGDFAQ AAQQLWLALR ALGRPLPTSH LDLACSLLWN661LIRHLLQRLW VGRWLAGRAG GLQQDCALRV DASASARDAA LVYHKLHQLH TMGKHTGGHL721TATNLALSAL NLAECAGDAV SVATLAEIYV AAALRVKTSL PRALHFLTRF FLSSARQACL781AQSGSVPPAM QWLCHPVGHR FFVDGDWSVL STPWESLYSL AGNPVDPLAQ VTQLFREHLL841ERALNCVTQP NPSPGSADGD KEFSDALGYL QLLNSCSDAA GAPAYSFSIS SSMATTTGVD901PVAKWWASLT AVVIHWLRRD EEAAERLCPL VEHLPRVLQE SERPLPRAAL HSFKAARALL961GCAKAESGPA SLTICEKASG YLQDSLATTP ASSSIDKAVQ LFLCDLLLVV RTSLWRQQQP1021PAPAPAAQGT SSRPQASALE LRGFQRDLSS LRRLAQSFRP AMRRVFLHEA TARLMAGASP1081TRTHQLLDRS LRRRAGPGGK GGAVAELEPR PTRREHAEAL LLASCYLPPG FLSAPGQRVG1141MLAEAARTLE KLGDRRLLHD CQQMLMRLGG GTTVTSSTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1177 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.