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SP110 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

SP110 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all SP110 antibodies →
$504.00each
Catalog Number: LS-G129999-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SP110 (NM_001185015), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
IFI41 | IFI75 | Phosphoprotein 41 | VODI | Phosphoprotein 75 | SP110 | SP110 nuclear body protein | Speckled 110 kDa | IPR1
Origin Species
Hu
NCBI GenBank
NM_004510
SwissProt
Q9HB58

Protein

Expression System
HEK293
Fusion Tag
FLAG, Myc
Molecular Weight
63.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MGRGFRMFTM TRAMEEALFQ HFMHQKLGIA YAIHKPFPFF EGLLDNSIIT KRMYMESLEA
61CRNLIPVSRV VHNILTQLER TFNLSLLVTL FSQINLREYP NLVTIYRSFK RVGASYEWQS
121RDTPILLEAP TGLAEGSSLH TPLALPPPQP PQPSCSPCAP RVSEPGTSSQ QSDEILSESP
181SPSDPVLPLP ALIQEGRSTS VTNDKLTSKM NAEEDSEEMP SLLTSTVQVA SDNLIPQIRD
241KEDPQEMPHS PLGSMPEIRD NSPEPNDPEE PQEVSSTPSD KKGKKRKRCI WSTPKRRHKK
301KSLPGGTASS RHGIQKKLKR VDQVPQKKDD STCNSTVETR AQKARTECAR KSRSEEIIDG
361TSEMNEGKRS QKTPSTPRRV TQGAASPGHG IQEKLQVVDK VTQRKDDSTW NSEVMMRVQK
421ARTKCARKSR LKEKKKEKDI CSSSKRRFQK NIHRRGKPKS DTVDFHCSKL PVTCGEAKGI
481LYKKKMKHGS SVKCIRNEDG TWLTPNEFEV EGKGRNAKNW KRNIRCEGMT LGELLKSGLL
541LCPPRINLKR ELNSK
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

555 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconj
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.