Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SLC2A9 / GLUT9 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G99253-100
Catalog Number: LS-G99253
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human GLUT9(SLC2A9) (NM_001001290), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Glucose transporter type 9 | GLUT-9 | GLUT9 | SLC2A9 | UAQTL2 | Glucose transporter 9 | GLUTX | URATv1
- NCBI GenBank
- NM_020041
- SwissProt
- Q9NRM0
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 55.5 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MKLSKKDRGE DEESDSAKKK LDWSCSLLVA SLAGAFGSSF LYGYNLSVVN APTPYIKAFY61NESWERRHGR PIDPDTLTLL WSVTVSIFAI GGLVGTLIVK MIGKVLGRKH TLLANNGFAI121SAALLMACSL QAGAFEMLIV GRFIMGIDGG VALSVLPMYL SEISPKEIRG SLGQVTAIFI181CIGVFTGQLL GLPELLGKES TWPYLFGVIV VPAVVQLLSL PFLPDSPRYL LLEKHNEARA241VKAFQTFLGK ADVSQEVEEV LAESRVQRSI RLVSVLELLR APYVRWQVVT VIVTMACYQL301CGLNAIWFYT NSIFGKAGIP LAKIPYVTLS TGGIETLAAV FSGLVIEHLG RRPLLIGGFG361LMGLFFGTLT ITLTLQDHAP WVPYLSIVGI LAIIASFCSG PGGIPFILTG EFFQQSQRPA421AFIIAGTVNW LSNFAVGLLF PFIQKSLDTY CFLVFATICI TGAIYLYFVL PETKNRTYAE481ISQAFSKRNK AYPPEEKIDS AVTDGKINGR PTRTRPLEQKLISEEDLAANDILDYKDDDDKV
511 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.