Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SLC11A2 / DMT1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G130003-100
Catalog Number: LS-G130003
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human DMT1(SLC11A2) (NM_001174130), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Divalent metal transporter 1 | Divalent cation transporter 1 | DMT-1 | NRAMP 2 | SLC11A2 | NRAMP2 | Dct-1 | DCT1 | DMT1
- Origin Species
- Human
- NCBI GenBank
- NM_000617
- SwissProt
- P49281
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 61.5 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MSTVDYLNSV SGDHGESASL GNINPAYSNP SLSQSPGDSE EYFATYFNEK ISIPEEEYSC61FSFRKLWAFT GPGFLMSIAY LDPGNIESDL QSGAVAGFKL LWILLLATLV GLLLQRLAAR121LGVVTGLHLA EVCHRQYPKV PRVILWLMVE LAIIGSDMQE VIGSAIAINL LSVGRIPLWG181GVLITIADTF VFLFLDKYGL RKLEAFFGFL ITIMALTFGY EYVTVKPSQS QVLKGMFVPS241CSGCRTPQIE QAVGIVGAVI MPHNMYLHSA LVKSRQVNRN NKQEVREANK YFFIESCIAL301FVSFIINVFV VSVFAEAFFG KTNEQVVEVC TNTSSPHAGL FPKDNSTLAV DIYKGGVVLG361CYFGPAALYI WAVGILAAGQ SSTMTGTYSG QFVMEGFLNL KWSRFARVVL TRSIAIIPTL421LVAVFQDVEH LTGMNDFLNV LQSLQLPFAL IPILTFTSLR PVMSDFANGL GWRIAGGILV481LIICSINMYF VVVYVRDLGH VALYVVAAVV SVAYLGFVFY LGWQCLIALG MSFLDCGHTV541SISKGLLTEE ATRGYVKTRTRPLEQKLISEEDLAANDILDYKDDDDKV
557 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.