Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SIPR3 / EDG3 / S1P3 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G100484-100
Catalog Number: LS-G100484
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human EDG3(S1PR3) (NM_005226), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- EDG-3 | EDG3 | LPB3 | S1P receptor EDG3 | S1P3 | S1PR3 | S1P receptor Edg-3 | S1P receptor 3
- Origin Species
- Hu
- NCBI GenBank
- NM_005226
- SwissProt
- Q99500
Protein
- Expression System
- HEK293
- Fusion Tag
- FLAG, Myc
- Molecular Weight
- 42.1 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MATALPPRLQ PVRGNETLRE HYQYVGKLAG RLKEASEGST LTTVLFLVIC SFIVLENLMV61LIAIWKNNKF HNRMYFFIGN LALCDLLAGI AYKVNILMSG KKTFSLSPTV WFLREGSMFV121ALGASTCSLL AIAIERHLTM IKMRPYDANK RHRVFLLIGM CWLIAFTLGA LPILGWNCLH181NLPDCSTILP LYSKKYIAFC ISIFTAILVT IVILYARIYF LVKSSSRKVA NHNNSERSMA241LLRTVVIVVS VFIACWSPLF ILFLIDVACR VQACPILFKA QWFIVLAVLN SAMNPVIYTL301ASKEMRRAFF RLVCNCLVRG RGARASPIQP ALDPSRSKSS SSNNSSHSPK VKEDLPHTAP361SSCIMDKNAA LQNGIFCNTRTRPLEQKLISEEDLAANDILDYKDDDDKV
378 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconj
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.