Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
RPS6KB2 / S6K2 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G100180-100
Catalog Number: LS-G100180
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human P70 S6 Kinase beta(RPS6KB2) (NM_003952), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- KLS | p70 S6K-beta | p70-beta-1 | p70-beta-2 | p70S6Kb | p70 ribosomal S6 kinase beta | p70(S6K)-beta | p70-beta | RPS6KB2 | S6K-beta | S6K-beta-2 | S6K-beta2 | SRK | p70-S6K 2 | S6 kinase-related kinase | p70 S6 kinase beta | p70 S6KB | p70S6K2 | S6 kinase 2 | S6K2 | STK14B
- Origin Species
- Hu
- NCBI GenBank
- NM_003952
- SwissProt
- Q9UBS0
Protein
- Expression System
- HEK293
- Fusion Tag
- FLAG, Myc
- Molecular Weight
- 53.3 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MAAVFDLDLE TEEGSEGEGE PELSPADACP LAELRAAGLE PVGHYEEVEL TETSVNVGPE61RIGPHCFELL RVLGKGGYGK VFQVRKVQGT NLGKIYAMKV LRKAKIVRNA KDTAHTRAER121NILESVKHPF IVELAYAFQT GGKLYLILEC LSGGELFTHL EREGIFLEDT ACFYLAEITL181ALGHLHSQGI IYRDLKPENI MLSSQGHIKL TDFGLCKESI HEGAVTHTFC GTIEYMAPEI241LVRSGHNRAV DWWSLGALMY DMLTGSPPFT AENRKKTMDK IIRGKLALPP YLTPDARDLV301KKFLKRNPSQ RIGGGPGDAA DVQRHPFFRH MNWDDLLAWR VDPPFRPCLQ SEEDVSQFDT361RFTRQTPVDS PDDTALSESA NQAFLGFTYV APSVLDSIKE GFSFQPKLRS PRRLNSSPRV421PVSPLKFSPF EGFRPSPSLP EPTELPLPPL LPPPPPSTTA PLPIRPPSGT KKSKRGRGRP481GRTRTRPLEQ KLISEEDLAA NDILDYKDDD DKV
513 aa
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconj
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.