LSBio
Rat Adiponectin Protein (Recombinant DDK (Flag))

Effect of Adiponectin (rat) (rec.) on the phosphorylation of acetyl-CoA carboxylase (ACC) in C2C12 cells. C2C12 myocytes were treated with 25 ug/ml for 15 minutes after serum starvation for 24 hours. 100 ug per well of total cell lysate was separated on reduced SDS-PAGE gels. Immunoblot analysis was performed to measure phosphorylation of ACC with an anti-phosphoACC antibody. 1: 25 ug/ml of Adiponectin (rat) (rec.). 2: 25 ug/ml of a FLAG-tagged control protein. 3: phosphate-buffered saline.

Rat Adiponectin Protein (Recombinant DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$451.00each
Catalog Number: LS-G3717-50

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Overview

Description
Rat adiponectin (aa 16-244) is fused at the N-terminus to a FLAG(R)-tag.
Category
Proteins & Peptides > Proteins > Recombinant Proteins
Alternative Names
Adiponectin | ADPN | ACDC | ADIPQTL1 | APM-1 | ADIPOQ | APM1 | ACRP30 | Gelatin-binding protein 28 | GBP28 | Gelatin-binding protein
Origin Species
Rat
NCBI GenBank
NM_004797|NP_004788.1
SwissProt
Q15848

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
~30kDa (SDS-PAGE)
Protein Type
Recombinant
Amino Acid Sequence
1aa 16-244

9 aa

Format

Purity
>90%
Conjugate/Label
Unconjugated
Endotoxin Level
< 0.1 EU/µg
Format
Liquid
Formulation
Lyophilized from PBS
Purity Measurement Method
SDS-PAGE (Coomassie Stain)

Storage & Handling

Storage Conditions
Store at 4°C for immediate use, or aliquot and store at -20°C for up to 3 months. Avoid freeze-thaw cycles.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.